Research Tevhnologist L3

University of Nebraska Medical Center•Omaha, NE

14h•Onsite

About The Position

This is a Researcher position to perform studies on mechanisms of alcohol-associated liver disease and the related comorbid condition of liver metastatic disease of colorectal cancer. The Researcher will be responsible for in vivo rodent models of alcohol-induced organ injury and in vitro approaches using cells lines and the isolation and culturing of primary hepatocytes and non-parenchymal liver cells. Additionally, independent participation in planning experimental designs, carrying out the procedures, and performing animal experiments using mouse models colorectal liver metastasis is expected. Will also be involved in the collection, analysis, and interpretation of experimental data, presentation of data at national meetings, manuscript preparation, and grant writing. Experiments with human and animal tissues will be done with in vitro experiments using routine cellular, molecular biology, and protein chemistry procedures. These routine lab procedures include microscopy, histology, immunohistochemistry, immunofluorescence, ELISA, flow cytometry, Western blot, quantitative PCR, molecular cloning, and miRNA analyses. Also, the Researcher will be responsible for assisting other individuals in the lab and will perform duties as assigned such as maintaining laboratory inventory and ordering needed supplies, preparing supplies for experiments, record-keeping, analyzing research data, assisting with the proper maintenance of lab safety documents, and overall compliance with regulatory requirements.

Requirements

3 years of experience
Proficiency in microscopy
Proficiency in histology
Proficiency in immunohistochemistry
Proficiency in immunofluorescence
Proficiency in ELISA
Proficiency in flow cytometry
Proficiency in Western blot
Proficiency in quantitative PCR
Proficiency in molecular cloning
Proficiency in miRNA analyses
Experience with in vivo rodent models
Experience with in vitro approaches using cell lines
Experience with isolation and culturing of primary hepatocytes and non-parenchymal liver cells
Experience with animal experiments using mouse models of colorectal liver metastasis
Ability to collect, analyze, and interpret experimental data
Ability to present data at national meetings
Ability to prepare manuscripts
Ability to assist with grant writing
Ability to maintain laboratory inventory and order supplies
Ability to prepare supplies for experiments
Ability to perform record-keeping
Ability to assist with lab safety documents
Ability to ensure compliance with regulatory requirements

Responsibilities

Perform studies on mechanisms of alcohol-associated liver disease and liver metastatic disease of colorectal cancer.
Conduct in vivo rodent models of alcohol-induced organ injury.
Utilize in vitro approaches with cell lines and isolation/culturing of primary hepatocytes and non-parenchymal liver cells.
Plan experimental designs.
Carry out experimental procedures.
Perform animal experiments using mouse models of colorectal liver metastasis.
Collect, analyze, and interpret experimental data.
Present data at national meetings.
Prepare manuscripts.
Engage in grant writing.
Perform routine cellular, molecular biology, and protein chemistry procedures including microscopy, histology, immunohistochemistry, immunofluorescence, ELISA, flow cytometry, Western blot, quantitative PCR, molecular cloning, and miRNA analyses.
Assist other individuals in the lab.
Maintain laboratory inventory and order supplies.
Prepare supplies for experiments.
Perform record-keeping.
Analyze research data.
Assist with the maintenance of lab safety documents.
Ensure overall compliance with regulatory requirements.