Research Technician
About The Position
The primary purpose of the position is to carry out cellular and molecular biology assays. This includes, but is not limited to, the passage of cell lines, flow cytometry assays, treatment of cell lines with small molecules, RNA isolation, quantitative-reverse transcription polymerase chain reaction experiments, protein isolation, ELISA and western blotting. The position will also entail assisting in the processing of biological samples from patients entering one of the UNC Kidney Center’s study cohorts. The individual will work in a collaborative network composed of physicians, PhDs, nurses, research personnel, and patients. ‘Hands on’ processing of biological samples (including blood and urine) is required. Sample processing includes but is not limited to cell isolation, DNA and RNA isolation, preparation and extraction of samples for gel electrophoresis, ELISA , Western blotting, flow cytometry, and other miscellaneous cellular, molecular and biochemical methods. The position will provide assistance organizing and storing reagents for sample processing. General laboratory duties such as cleaning, autoclaving, and preparation of stock solutions will be required as well. All requirements for laboratory safety in handling blood borne pathogens and HIPAA compliance must be kept current.
Requirements
- Laboratory safety in handling blood borne pathogens
- HIPAA compliance
Responsibilities
- Carry out cellular and molecular biology assays
- Passage of cell lines
- Perform flow cytometry assays
- Treat cell lines with small molecules
- Perform RNA isolation
- Conduct quantitative-reverse transcription polymerase chain reaction experiments
- Perform protein isolation
- Conduct ELISA and western blotting
- Assist in the processing of biological samples from patients
- Perform 'hands on' processing of biological samples (including blood and urine)
- Isolate cells
- Isolate DNA and RNA
- Prepare and extract samples for gel electrophoresis, ELISA, Western blotting, and flow cytometry
- Perform other miscellaneous cellular, molecular and biochemical methods
- Assist organizing and storing reagents for sample processing
- Perform general laboratory duties such as cleaning, autoclaving, and preparation of stock solutions
- Maintain current laboratory safety requirements in handling blood borne pathogens and HIPAA compliance
Stand Out From the Crowd
Upload your resume and get instant feedback on how well it matches this job.
What This Job Offers
Job Type
Full-time
Career Level
Mid Level
Education Level
No Education Listed
