Research (Professional)

University of Pittsburgh • Pittsburgh, PA

3d

About The Position

Research Technician position needed for the Dr Ait ALi lab within Ophthalmology Department.

Requirements

• General knowledge about antibody selection, gene target selection, cell culture in the scale of tens to hundred million cells for quantitative profiling experiments, cell harvest, lysis, protein extraction, solubilization, fractionation and protein biochemistry.
• Laboratory techniques: competent cells, agar plates, plasmid preparation, plaque assay.
• Bacteriophage detection, and preparation of aliquots, common buffers and bacterial grow media.
• Cell line maintenance, passaging, cryostorage, and harvesting; aliquot preparation, mycoplasma test.
• Model organism: mouse (Mus musculus), retina extraction and embedding in OCT compound, ear punching and tailing of mice. Dissection of organs and cryostorage. Injections (intracardiac, subcutaneous, intraperitoneal, retroorbital), anesthesia, euthanasia, PCR for genotyping.
• Western blot, BCA assay, Ca measurement assay.
• Protein extraction from cells, quantification, trypsin digestion.
• Nucleic acids extraction, purification and quantification, PCR, cDNA synthesis, RT-PCR.
• Software/Databases Excel, Image J, NCBI, Ensemble, Primer 3, Prism, Photoshop. Adobe Illustrator, snapgene.

Nice To Haves

• Bachelor's degree preferred

Responsibilities

• Bibliographic research and experimental design to validate scientific hypothesis
• General laboratory organization: writing protocols, inventories, purchases, safety documentation
• Design and implementation of experimental workflows. Troubleshooting of different technical and biological parameters over multi-day experiments. Validation of methods and design of experimental condition comparisons.
• Learning a method to optimize laboratory techniques.
• General knowledge about antibody selection, gene target selection, cell culture in the scale of tens to hundred million cells for quantitative profiling experiments, cell harvest, lysis, protein extraction, solubilization, fractionation and protein biochemistry.
• General laboratory organization: writing protocols, inventories, purchases, safety documentation
• Laboratory techniques: competent cells, agar plates, plasmid preparation, plaque assay.
• Bacteriophage detection, and preparation of aliquots, common buffers and bacterial grow media.
• Cell line maintenance, passaging, cryostorage, and harvesting; aliquot preparation, mycoplasma test.
• Model organism: mouse (Mus musculus), retina extraction and embedding in OCT compound, ear punching and tailing of mice. Dissection of organs and cryostorage. Injections (intracardiac, subcutaneous, intraperitoneal, retroorbital), anesthesia, euthanasia, PCR for genotyping.
• Western blot, BCA assay, Ca measurement assay.
• Protein extraction from cells, quantification, trypsin digestion.
• Nucleic acids extraction, purification and quantification, PCR, cDNA synthesis, RT-PCR.

What This Job Offers

• Career Level: Entry Level