Research Technician IV: Gurnett Lab

Children’s Hospital of Philadelphia•Philadelphia, PA

23h

About The Position

This role participates in a planned research project by performing research protocols. Works independently and self-directed as assigned, providing oversight and technical training to other research staff. Needs a specialized education in the biological or chemical sciences and is required to consistently exercise discretion and judgment in execution, interpretation of experiments, and of data derived. We are seeking a highly motivated, detail-oriented Research Technician to join our laboratory focused on stem cell biology and neurological disease research. The successful candidate will support ongoing projects involving human iPSC-derived cells and a range of molecular biology and cell-based assays.

Requirements

Bachelor's Degree Required
At least three (3) years of classroom and directly relevant and progressive (increasingly complex/ advanced) lab experience Required
Advanced understanding of lab procedures, protocols, and equipment. (Required proficiency)
Advanced proficiency in various research and/or analytical techniques. (Required proficiency)
Basic proficiency in experiment design and troubleshooting (Required proficiency)
Excellent verbal and written communications skills (Required proficiency)
Excellent organizational skills (Required proficiency)
Excellent critical thinking / problem-solving skills (Required proficiency)
Excellent analytical skills (Required proficiency)

Nice To Haves

Bachelor's Degree in Biology, Molecular Biology, Biochemistry or a related field Preferred
Master's Degree Preferred
Previous lead or supervisory experience in a research lab environment Preferred
Hands-on experience with mammalian cell culture (stem cell experience strongly preferred)
Experience with DNA/RNA extraction and PCR-based techniques
Experience with immunocytochemistry
Experience working with human iPSC-derived neurons or other differentiated cell types
Broader experience in molecular biology techniques
Proficiency in Microsoft Excel and PowerPoint
Highly motivated, reliable, and a quick learner with the ability to work both independently and as part of a team
Basic data analysis and quantitative skills

Responsibilities

Perform routine and advanced mammalian cell culture, including human iPSC maintenance and differentiation
Conduct DNA and RNA extraction from cultured cells and tissue samples
Perform PCR, RT-qPCR, and other molecular biology assays
Conduct immunocytochemistry and imaging-based analyses
Maintain accurate laboratory records and ensure proper documentation of experiments
Organize, analyze, and present experimental data using Excel and PowerPoint
Assist with reagent preparation, inventory management, and general laboratory maintenance
Support time-sensitive stem cell experiments, including occasional weekend work when required
Independently performs advanced, complex research procedures on experimental samples
Formulates and performs complex lab experiments and analyzes/ reports the data, including interpretation of methodologies
Independently performs tasks that require knowledge of advanced techniques and lab equipment
Performs advanced calculations and maintains appropriate log and lab notebooks
Plans and modifies research protocols and procedures with minimal direction from the principal investigator, supervisor or other designee
Assists in development & troubleshooting of new techniques and ideas
Prepares laboratory reagents, monitors and maintains laboratory inventory
Supervises, trains and mentors junior and new staff members
Determines staff work assignments, schedules and laboratory priorities as directed
Presents & discusses work at weekly lab meetings and national symposia
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Assists in writing of grants, journal articles, posters and oral presentations