Research Technician II - Pediatrics Genetics

Washington University in St. Louis • St. Louis, MO

1d • $17 - $26 • Onsite

About The Position

The Greer lab is interested in how non-genetic information can be transmitted across generations and how non-genetic information regulates complex phenotypes. An increasing number of complex phenotypes, such as physical appearance, energy metabolism, psychological state, and longevity, have recently been shown to be regulated, in part, by epigenetic information. Epigenetics describes how gene expression changes occur without changes to the DNA sequence. Proteins, RNA molecules, or chemical modifications to histones or DNA can induce these epigenetic changes. How this information, which is not directly coded in our DNA, is passed from generation to generation is still unknown. Understanding the molecular determinants of stable epigenetic memory will provide insight into how environmental changes can affect the health and lifespan of not only the individual who experiences them, but also of their progeny. Our goals are to identify epigenetic inheritance phenotypes and to elucidate the mechanisms behind their transmission across generations. We also aim to understand how epigenetic mis-regulation can cause developmental defects and disease. In the Greer lab we use the nematode C. elegans, the social amoeba Dictyostelium, mice, and mammalian tissue culture systems to study how non-genetic information can be transmitted across generations as well as to understand the role of non-genetic information in regulating a variety of biological processes. We use a wide variety of genetic, biochemical, and molecular techniques to decipher these complex questions. Some experiments would entail worm, Dictyostelium, or mouse husbandry, extracting gDNA and proteins from worm and tissue samples, running dot blots and western blots, purifying proteins and testing their enzymatic activity. Assists with the technical aspects of studies and experiments, including documentation and preparation of materials. The Greer lab strives to be a place of fun, collegiality, and collaboration.

Requirements

• Bachelor’s degree or combination of education and/or experience may substitute for minimum education.
• No specific work experience is required for this position.
• The ideal candidate will have up to one year of experience in a laboratory setting.

Nice To Haves

• Prior research experience with any of the following: tissue culture, C. elegans, Dictyostelium discoideum, or mice, would be preferred for this position.

Responsibilities

• Assists with research studies, experiments and assays, including collection of data, preparation of solutions and setup and maintenance of equipment.
• Performs data entry and maintains data files on research.
• Prepares tissue cultures, reagents, probe tests, etc.
• Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
• Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
• Assists with general lab maintenance and cleaning.
• Performs other duties as assigned.

Benefits

• Up to 22 days of vacation, 10 recognized holidays, and sick time.
• Competitive health insurance packages with priority appointments and lower copays/coinsurance.
• Free Metro transit U-Pass for eligible employees.
• Defined contribution (403(b)) Retirement Savings Plan, which combines employee contributions and university contributions starting at 7%.
• Wellness challenges, annual health screenings, mental health resources, mindfulness programs and courses, employee assistance program (EAP), financial resources, access to dietitians, and more!
• 4 weeks of caregiver leave to bond with your new child.
• Family care resources are also available for your continued childcare needs.
• Adult care resources.
• Tuition for you and your family, including dependent undergraduate-level college tuition up to 100% at WashU and 40% elsewhere after seven years with us.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level