Research Technician II- Cellular and Molecular Therapeutics

Children’s Hospital of Philadelphia•Philadelphia, PA

1d•$43,900 - $53,440•Onsite

About The Position

This entry-level research technical role participates in a planned research projects by performing research protocols, experimental procedures and other laboratory tasks under direct supervision. May occasionally exercise minimal discretion and judgment in execution and interpretation of experiments, and of data derived. The Raymond G. Perelman Center for Cellular and Molecular Therapeutics (CCMT) is dedicated to the understanding, development, and application of gene and related cell and nucleic acid therapies and the promotion of professional and public education in the field. The CCMT fosters a multidisciplinary approach among researchers and scientists working to discover new gene and cell therapies in search of cures for debilitating and life-threatening disorders. The Davidson Laboratory, an integral part of the CCMT, studies neurodegenerative disorders with the goal of finding long term therapies. We are looking for a research technician to work alongside scientists investigating neurological developmental and degenerative diseases. The ideal candidate will have laboratory experience and knowledge of molecular biology. Familiarity with basic laboratory techniques such as PCR, gel electrophoresis, and DNA/RNA isolation desired. The technician will be trained in next generation sequencing library preparation, tissue culture work, molecular cloning, and proteomics such as western blotting. Prior experience in any of these specialties is a plus.

Requirements

At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Required
Intermediate understanding of lab procedures, protocols, and equipment.
Basic exposure to lab specific experiments / techniques and scientific analysis
Excellent verbal and written communications skills
Excellent organizational skills
Solid critical thinking / problem-solving skills
Solid analytical skills

Nice To Haves

Associate's Degree Preferred
Bachelor's Degree Preferred
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Preferred
Prior experience with cell culture and cloning Preferred
Familiarity with basic laboratory techniques such as PCR, gel electrophoresis, and DNA/RNA isolation desired.
Prior experience in any of these specialties is a plus.

Responsibilities

Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
Performs research procedures on experimental samples as directed
Records and checks experiments/ results for completeness and accuracy
Performs calculations and maintains appropriate log and lab notebooks
Performs tasks that require familiarity with fundamental techniques and lab equipment
Perform tissue culture work with various cell lines
Prepare next generation sequencing libraries
Perform molecular cloning
Perform protein analysis
May assist with planning specific research protocols and procedures
Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
Assists in care and maintenance of lab equipment
May coordinate schedules of tests and procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
Conducts literary searches as directed