Research Technician, Biochemistry, Brown

Vanderbilt University • Nashville, TN

2d • Onsite

About The Position

The Brown Laboratory focuses on understanding defects in macromolecular protein assembly that underlie human disease. Specifically, using X-ray crystallography and related techniques, we seek to solve the 3-D structures of the individual protein components of large assemblies known to be critical for proper mitochondrial physiology; defects in which cause severe diseases ranging from anemia to neurodegeneration. Ultimately, we can use this detailed molecular information to uncover how genetic mutations cause changes in protein folding, leading to aberrant assembly and subsequent human disease. In addition to X-ray crystallography, the laboratory also employs a wide range of biochemical and biophysical techniques to complement our structural work, including mass spectrometry, fluorescence-based enzyme assays, analytical ultracentrifugation, and various binding assays, to name few. Members of the laboratory will be based in the recently-renovated space and will work in a collaborative and interactive community space shared by other biochemistry laboratories.

Requirements

• A Bachelor’s degree in biochemistry, biology, chemistry, or a related field from an accredited institution of higher education is necessary.
• Ability to adhere to environmental health and safety standards is necessary.
• Ability to adhere to scientific integrity standards in data collection and reporting is necessary.

Nice To Haves

• Evidence of excellence in laboratory coursework is preferred.
• Experience in an academic biochemistry laboratory is strongly preferred.
• Experience in a college or university academic laboratory (e.g. post-baccalaureate program or summer internship) is preferred.
• Experience in PCR/cloning is strongly preferred.
• Experience in protein expression and purification is strongly preferred.
• Ability to understand and follow scientific research protocols and procedures is strongly preferred.
• Proficiency in written and oral scientific communication is preferred.
• Ability to work in a collaborative environment and take direction is strongly preferred.
• Ability to analyze and interpret scientific data is preferred.

Responsibilities

• Making common laboratory stocks, media, buffers, etc.
• Carrying out tasks related to protein expression (e.g. PCR, plasmid preparation, bacterial cell culture growth)
• Maintaining laboratory equipment
• Cleaning and autoclaving glassware and equipment
• Assisting with monitoring and ordering supplies and receiving packages
• Attendance at regular meetings with Principal Investigator to discuss laboratory tasks and data
• Attendance and participation in regular group laboratory meetings
• Proficiency and accuracy in maintaining lab notebook and documentation
• Assisting Principal Investigator in training other researchers in lab procedures and protocols

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level