UNIV - Research Specialist I - Department of Regenerative Medicine

Medical University of South Carolina•Charleston, SC
•$37,944 - $60,690

About The Position

The Department of Regenerative Medicine at the Medical University of South Carolina is hiring a full-time Research Specialist I for a basic science research laboratory focused on cardiac and connective tissue diseases. The position works under the general supervision of Dr. Norris and senior lab personnel and is suited to a motivated, well-organized individual with strong communication skills who enjoys contributing to a team. The Research Specialist I performs histological and molecular biology techniques, supports mouse colony husbandry and tissue collection, and conducts cell culture and organoid work. The role also includes laboratory maintenance, inventory and equipment upkeep, animal and experimental recordkeeping, protocol support, computer backup, and statistical analysis. The position requires learning and using new processes, tools, and equipment, and may require rotating shifts and overtime.

Requirements

  • High school diploma and two years of work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or another natural, life, or health care science may substitute for the two years of experience.
  • Ability to work rotating shifts and overtime as required.
  • Ability to learn and use new processes, tools, and equipment as required.
  • Ability to meet the listed physical requirements, including lifting and moving specified materials and equipment, performing repetitive fine-motor tasks, working in confined spaces and temperature extremes, and being physically qualified for respirator use.

Nice To Haves

  • Previous laboratory experience is helpful but not required.

Responsibilities

  • Perform tissue processing, embedding, sectioning, immunostaining, and microscopy, including acquiring micrographs.
  • Fix tissue and/or cells and embed tissue in paraffin or OCT before sectioning.
  • Work with lab members to provide husbandry for transgenic and targeted mouse lines, including genotyping, weaning, line maintenance, and general care.
  • Assist with mouse dissection, tissue collection, and tissue processing.
  • Perform DNA isolation, PCR, and agarose gel electrophoresis; extract RNA, synthesize cDNA, and run qRT-PCR.
  • Conduct cell culture experiments and maintain cell cultures.
  • Generate gastrointestinal organoids from tissue and maintain intestinal organoids in vitro.
  • Wash glassware, prepare solutions, organize the laboratory, track essential stocks, and help create a reagent and lab-item inventory.
  • Maintain equipment, including scale and pipette accuracy.
  • Maintain accurate animal and experimental records; assist with writing protocols and optimizing experiments.
  • Back up computers, perform statistical analyses, and record experimental protocols and data.
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