Research Lab Technician - Neuroscience

Mass General Brigham•Boston, MA

15h•Onsite

About The Position

Responsible for maintaining laboratory equipment, organizing and preparing samples, conducting experiments, and recording and analyzing data. Candidates who are in the process of completing their bachelor's degree have a grace period of six months from their hire date (up to 1 year if starting per diem) to provide degree equivalency verification. We are a neuroscience laboratory at Mass Eye and Ear/Harvard Medical School studying developmental mechanisms that underlie generation of functionally diverse neurons in the mouse auditory system. To that end, we utilize a range of approaches, including advanced microscopy, single-cell genomics, molecular genetics, and machine learning. We are looking for a Research Assistant to participate in various projects in the laboratory. Working under direct supervision of the principal investigator, the candidate will be responsible for: 1) handling live mice to set up crosses, wean litters, collect tissue biopsies for genotyping, and inject various drugs 2) extracting, dissecting, processing and sectioning cochlear and brain tissues from euthanized mice 3) detecting mRNA and proteins of interest by in situ hybridization and immunohistochemistry 4) preparing buffers and reagents for molecular biology 5) performing gel electrophoresis 6) acquiring images using epifluorescence and confocal microscopy 7) assisting in general laboratory maintenance, including setting up of equipment, and organizing reagents and supplies. In addition, opportunities will be available to conduct gene expression analysis using R or Python in collaborative projects. Offering a broad exposure to standard experimental and analytical approaches employed in a molecular neuroscience lab, this position is ideal for individuals seeking to pursue graduate studies in biomedical sciences or medicine.

Requirements

Bachelor's Degree Related Field of Study required
Prior laboratory experience, either through academic coursework, internships, or employment
Strong theoretical and practical knowledge of laboratory techniques and procedures.
Familiarity with common laboratory equipment and instruments used in research experiments.
Proficiency in data collection, analysis, and scientific software tools.
Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively.
Strong problem-solving skills and the ability to troubleshoot technical issues.
Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges.
Bachelor's degree in neuroscience, genetics, biology or a related discipline with at least six months of experience in a wet lab environment is required.
Comfort with handling live mice and performing procedures on anesthetized or euthanized mice is required.

Nice To Haves

Demonstrated abilities in relevant laboratory techniques preferred.

Responsibilities

Perform laboratory experiments following established protocols and procedures.
Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines.
Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed.
Record experimental data accurately and maintain organized records of procedures, protocols, and results.
Compile and analyze data using appropriate statistical and analytical methods.
Assist in data interpretation and presentation, contributing to research findings and reports.
Handling live mice to set up crosses, wean litters, collect tissue biopsies for genotyping, and inject various drugs.
Extracting, dissecting, processing and sectioning cochlear and brain tissues from euthanized mice.
Detecting mRNA and proteins of interest by in situ hybridization and immunohistochemistry.
Preparing buffers and reagents for molecular biology.
Performing gel electrophoresis.
Acquiring images using epifluorescence and confocal microscopy.
Assisting in general laboratory maintenance, including setting up of equipment, and organizing reagents and supplies.
Conduct gene expression analysis using R or Python in collaborative projects.