Research Lab Technician II - OHNS Cancer Research

Mass General Brigham•Boston, MA

4d•$24 - $39•Onsite

About The Position

Under the direction of the Principal Investigator, the Research Technician II will support preclinical and translational cancer research within the Department of Otolaryngology–Head and Neck Surgery at Mass Eye and Ear. The position is based in the research space at SERI, 50 Staniford Street, Boston, under the supervision of Dr. Rahul Pal. The technician will play a central role in maintaining and executing in vivo mouse tumor studies and associated cellular and molecular biology experiments. A strong foundation in animal handling, cell biology, and standard molecular biology techniques is essential. This position is well suited for a candidate interested in applying rigorous biological methods to translational cancer research, with opportunities to learn and grow into cutting edge imaging and computational analysis approaches developed in the laboratory.

Requirements

Bachelor of Science degree in Biology, Biochemistry, Biomedical Engineering, or a related field is required
1-2 years of relevant post-baccalaureate laboratory experience
Demonstrated hands-on experience with animal models and/or a strong background in cell culture and molecular biology techniques, including tissue handling, mammalian cell culture, and standard biochemical assays
Strong proficiency in following experimental protocols with precision
Maintaining accurate records
Working with biological samples
Excellent organizational skills
Attention to detail
Communication skills
Ability to work effectively as part of a collaborative research team
Ability to manage multiple tasks
Ability to adapt to shifting experimental priorities in a fast-paced research environment

Nice To Haves

Prior experience with mouse tumor models or cancer biology research
Familiarity with molecular biology techniques such as Western blotting, nucleic acid extraction, flow cytometry, immunofluorescence, and immunohistochemistry
Exposure to biomedical imaging, quantitative assays, or interdisciplinary research environments integrating biology and technology
Experience with programming languages such as Python or MATLAB
Strong interest in transitioning into a highly interdisciplinary program that integrates cancer biology, molecular imaging, and computational analysis

Responsibilities

Establishing, maintaining, and monitoring murine tumor models
Performing tumor implantation, measurements, and longitudinal study support
Handling and processing of mouse tissues following in vivo experiments
Supporting ex vivo tissue preparation and analysis, including sample preservation for downstream assays
Maintaining routine mammalian cell culture
Establishing and expanding primary cell lines from tumor or tissue specimens
Performing cell-based assays to evaluate biological responses to therapeutic or imaging agents
Supporting experimental design and execution of mechanistic studies in vitro
Tissue processing for downstream molecular analyses
Extraction of DNA, RNA, and proteins from tissue and cell samples
Western blotting, immunodetection, and related protein analysis techniques
Flow cytometry and sample preparation for immune and tumor cell profiling
Supporting downstream data interpretation in collaboration with investigators
Participation in ex vivo tissue imaging and fluorescence-based assays
Exposure to advanced fluorescence lifetime imaging and other optical imaging modalities developed in the laboratory
Assisting with instrument testing, validation, and experimental imaging workflows
Performing data analysis using commercial and laboratory-developed software tools
Exposure to image processing and quantitative analysis pipelines
Maintaining accurate experimental records, databases, and sample inventories
Assisting with preparation of reports, figures, manuscripts, and presentations
Supporting regulatory documentation and compliance requirements as needed
Working collaboratively with multidisciplinary team members across basic and clinical research environments