Research Lab Ops Manager Microscopy Core

Mass General Brigham•Belmont, CA

6h•Onsite

About The Position

The Research Lab Operations Manager specialized in Microscopy Core; Researcher in the Translational Neuroscience Lab (TNL). Performs a variety of research project as part of the TNL and research administration activities in direct support of the Department's research faculty and programs. As a researcher in the TNL, projects will include investigations on the molecular and cellular biology of psychiatric disorders and psychiatric symptoms in neurodegenerative disorders. Assays used include a range of microscopy techniques, multiplex protein and RNA measurements and cell culture assays from human postmortem brain tissue. As the Manager of the Microscopy core, responsibilities include managing the overall functioning of the research operation. Serves as a front-line resource for researchers in the Department, acting as liaison to internal administrative units and external sponsors on matters relating to research programs. This role involves specialized expertise in advanced microscopy techniques, including transmission electron microscopy (TEM), laser scanning confocal microscopy (LSCM), and two-photon microscopy. The position requires in-depth knowledge to guide study design, data collection, and analysis, as well as budgeting for research projects. Responsibilities include training and supporting researchers in these techniques, overseeing instrument maintenance and usage scheduling, and managing the financial and operational aspects of the Core Facility. The microscopist will also supervise on-site contracted services to ensure equipment remains in optimal working condition.

Requirements

Bachelor's Degree Related Field of Study required or Master's Degree Related Field of Study preferred
Research experience (in a lab setting) 3-5 years required
Must be able to make independent, effective decisions in appropriate situations.
Must possess a scientific imagination commensurate with the independent execution of research projects.
Must have an aptitude for technical problem solving.
Excellent organizational skills to ensure smooth functioning of lab.
Demonstrated analytical skills and ability to logically sequence experiments to maximize research.
Must possess an aptitude for budget management.

Nice To Haves

Hands-on experience in biological (structural and immune-) TEM, including resin embedding, ultrathin sectioning, staining, image acquisition and processing
Hands-on experience with standard light microscopy, wide-field fluorescence microscopy, and confocal microscopy
Experience in, or ability to learn, flow cytometry and fluorescence activated cell sorting
Familiarity with basic image processing tools and methods
Ability to effectively communicate and professionally interact with a diverse population of scientific researchers, administrative and facilities staff, service engineers, and sales reps.
Ability to understand electron and optical microscope design and perform hands-on troubleshooting as needed
Patient, responsible, and service-oriented demeanor
Excellent communication skills
Competent computer skills in order to use a variety of electronic systems

Responsibilities

Responsible for oversight of all lab supply and equipment, purchasing and maintenance.
Coordinates all regulatory and compliance activities.
Trains staff in specialized lab techniques.
Assists with subject scheduling.
Provides input on strategy, based on knowledge of industry and trends.
Applies project management techniques to ensure development initiatives stay on track.
Contributes to scientific literature and may present the data at meetings.
Carries out independent research projects which involve the execution of highly sophisticated techniques.
Performs other duties as assigned
Complies with all policies and standards
Maintain the Reichert-Jung ultramicrotome and ancillary TEM instrumentation.
Oversee and maintain the JEOL JEM1200EX TEM and cooling systems.
Conduct TEM for MRC and external investigators, including: Resin sample preparation, ultramicrotomy, staining, data collection, and image processing.
Immuno-electron microscopy techniques.
Cryo-preservation and freeze substitution of samples.
Electron tomography on ultrathin sections and tomogram reconstruction.
Negative staining for specific biological samples.
Prepare sample supports (Formvar and carbon-coated grids, glow discharge, addition of gold fiducials).
Supervise on-site contracted TEM services.
Train users on independent operation and image processing tools for the confocal microscope.
Provide technical assistance and advice for confocal microscopy as needed.
Perform confocal microscopy for MRC and external investigators.
Maintain the Leica SP8-TCS LSCM in optimal condition.
Supervise on-site contracted services for LSCM.
Train users on the two-photon microscope and associated image processing tools.
Provide technical guidance and assistance for two-photon microscopy.
Maintain the two-photon microscope in optimal condition.
Supervise on-site contracted services related to two-photon microscopy.
Implement and manage a structured user sign-up system for all instruments.
Ensure compliance with all lab safety, security, and hazardous waste disposal regulations.
Maintain an adequate inventory of lab consumables and essential supplies.
Generate and issue monthly usage invoices for all Core instruments and services.
Prepare and manage the annual budget for Core expenses.
Keep detailed and accurate records of all financial transactions associated with the Core facility.