About The Position

This position serves as a Without Compensation Research Biological Laboratory Technician (Animal) within the Office of Research and Development (ORD). The VA Research Program works to promote Veteran-centered care, improve patient experiences and outcomes across VA healthcare and community settings, and advance value-driven care by providing Veterans high-quality care at the lowest financial burden. The position is unpaid and has an intermittent work schedule. The technician performs laboratory procedures involving biospecimen handling and storage; nucleic acid and protein preparation; molecular biology, cell culture, viral vector, CRISPR/Cas9, and flow cytometry techniques; rodent husbandry, genotyping, and experimental procedures; and sample preparation and analysis. The role also includes use of specified computer software, general laboratory organization and maintenance, and hazardous waste handling and disposal.

Requirements

  • U.S. citizenship is required.
  • Selective Service registration is required for males born after December 31, 1959.
  • Applicants are subject to a background/security investigation.
  • Selected applicants must complete online onboarding and provide acceptable identification for pre-employment requirements.
  • Participation in the seasonal influenza vaccination program is required for Department of Veterans Affairs Health Care Personnel.
  • Meet the qualifications by the announcement closing date of 11/02/2026 through one of the listed pathways: specialized experience, education, or a combination of education and experience.
  • Specialized experience pathway: at least one year of full-time duties performing and/or assisting with laboratory experiments listed in the major duties; prior biomedical research activity involving stress signaling in eukaryotes; and authorship on research manuscripts reflecting meaningful contributions through direct participation in planning, execution, and completion of research objectives for a program.
  • Education pathway: successful completion of a full four-year course of study leading to a bachelor's degree with a major or at least 24 semester hours in qualifying scientific or technical courses; at least 6 semester hours must be directly related to the position. A full course of study of at least 12 months at a school for clinical laboratory technicians may be substituted for specialized experience.
  • Combination pathway: applicants may combine education and experience to qualify.
  • Education used to qualify must be from an institution accredited by an accrediting institution recognized by the U.S. Department of Education; foreign education requires a Certificate of Foreign Equivalency with the transcript.
  • The work involves prolonged standing and lifting moderately heavy items.

Responsibilities

  • Handle and store biospecimens, including liquid nitrogen cold storage.
  • Prepare RNA, DNA, and protein from mammalian cell and rodent samples.
  • Perform recombinant DNA techniques, including PCR, cloning, transformation, mini and maxi DNA preparations, rtPCR studies, and next-generation sequencing library generation and analysis.
  • Perform protein techniques, including cell extract preparation, quantification, electrophoresis, immunoblotting, and imaging.
  • Culture, propagate, cryopreserve, and stain mammalian cells.
  • Construct, generate, and use lentiviral and adeno-associated vectors in cell culture and rodent models.
  • Perform CRISPR/Cas9 gene targeting in mammalian cells.
  • Isolate mammalian cells using flow cytometry or related technologies.
  • Conduct mouse and rat husbandry, including weaning, ear tagging and punching, breeding, and euthanasia.
  • Perform mouse and rat genotyping, including genomic DNA preparation, PCR genotyping, and data entry.
  • Conduct experimental protocols involving rodent models, including tissue sampling.
  • Prepare and analyze histological samples from rodent models.
  • Use computer software including ImageJ, Prism, Microsoft Office, Python, RStudio, SQL, and AI models such as ChatGPT.
  • Maintain general laboratory organization, maintenance, and function.
  • Handle and dispose of hazardous waste.
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