Research Associate - RI Kid & Urology Baker

Nationwide Children's Hospital • Columbus, OH

35d

About The Position

Research Assoc, Research Institute at Nationwide Children’s Hospital, Columbus, OH. Conduct research on genetic mechanisms underlying Prune Belly Syndrome (PBS) & posterior cloaca malformations. Establish & maintain patient-derived iPSC biobank, incl reprogramming, maintenance & characterization of iPSCs using immunofluorescence, flow cytometry, RT-PCR & Western blot to assess pluripotency. PBMC & urine sample processing from patients & families for downstream analysis & biobanking. Perform molecular cloning & site-directed mutagenesis for plasmid generation. Resp for general lab maintenance. The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the individuals so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct, and control the work of employees under their supervision. EOE M/F/Disability/Vet

Requirements

• Bachelor’s in Bio, Biotech or Microbio
• 1 yr exp in molecular bio techniques (RNA, DNA & protein extraction, cDNA synthesis & RT-PCR)
• immunofluorescence (mouse tissues, imaging, cryosectioning & mouse handling)
• ELISA & cell culture techniques

Responsibilities

• Conduct research on genetic mechanisms underlying Prune Belly Syndrome (PBS) & posterior cloaca malformations
• Establish & maintain patient-derived iPSC biobank, incl reprogramming, maintenance & characterization of iPSCs using immunofluorescence, flow cytometry, RT-PCR & Western blot to assess pluripotency
• PBMC & urine sample processing from patients & families for downstream analysis & biobanking
• Perform molecular cloning & site-directed mutagenesis for plasmid generation
• Responsible for general lab maintenance

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level