Research Associate I

Gladstone Companies • San Francisco, CA

6d • $60,147 - $69,307

About The Position

The Bruneau Lab at Gladstone Institutes is seeking a Research Associate to study gene regulation during heart development using mouse models in vivo and stem cells in vitro. The role primarily focuses on culturing induced pluripotent stem cells (iPSCs) and performing molecular biology techniques to understand gene expression regulation during in vitro cardiac differentiation. Candidates should be motivated and able to work well on a team. The posting describes potential for one or two publications within a two-year period and encourages applicants considering graduate, medical, or dental school.

Requirements

• Associate's degree and at least 2 years of relevant experience.
• Excellent verbal and written communication skills.
• Strong work ethic.
• Strong organizational skills, attention to detail, and ability to multitask.
• Excellent communication and interpersonal skills, including the ability to work effectively in a team setting.

Nice To Haves

• BA/BS in biological sciences and at least 6 months of practical experience in a research laboratory.
• Experience with iPSC or cell culture.
• Experience with flow cytometry.
• Molecular biology skills such as PCR, western blotting, and RT-qPCR.
• Laboratory management experience, including ordering, restocking, or database maintenance.
• R or Python programming, or equivalent.
• Experience with next-generation sequencing, such as RNA-seq or ChIP-seq.
• CRISPR/Cas9 genome-editing experience.
• Proficiency in Excel, Word, ImageJ Fiji, Photoshop, and Illustrator.

Responsibilities

• Maintain and expand iPSC lines, including routine passaging, freezing, and thawing.
• Apply differentiation protocols to generate cardiomyocytes consistently and reproducibly, and assess differentiation efficiency using flow cytometry.
• Perform PCR, RT-qPCR, and western blotting to analyze gene expression and protein levels.
• Use RNA-seq, ChIP-seq, and ATAC-seq to investigate gene regulation and epigenetic changes in iPSC-derived cardiomyocytes.
• Analyze experimental and sequencing data and interpret results.
• Update records and log books and assist in maintaining laboratory records and inventory.
• Assist with laboratory management, including ordering reagents and organizing inventory.

Benefits

• Competitive salary commensurate with education and experience.
• Medical, dental, and vision benefits.
• Retirement plan.
• Paid vacation.
• Commuter benefits.
• Access to free shuttle transportation.
• Collaborative, inclusive culture and resources for professional growth and learning.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Associate degree