Research Assistant

Harvard University • Boston, MA

15d • Onsite

About The Position

The Yellen lab at Harvard Medical School Neurobiology is looking for a research technician. Our research is focused on understanding neuronal metabolism and how it is influenced by – and influences – neuronal activity and excitability. The lab develops tools for studying neural metabolism, including engineered proteins that function as fluorescent biosensors of metabolism, and we study the dynamics of metabolism in mouse brain using both ex vivo and in vivo imaging methods. We also work to learn the role of altered metabolism in preventing epileptic seizures, inspired by the ability of ketogenic diets to treat human epilepsy.

Requirements

• At least one-year related work experience (relevant course work may count towards experience)
• College background or equivalent work experience, preferably in a related discipline
• General education in molecular biology
• Familiarity with basic concepts of plasmids, expression vectors, and subcloning
• Hands-on experience with good laboratory practices and sterile technique
• Attention to detail
• Creative problem solving

Nice To Haves

• Some experience with mice is preferred

Responsibilities

• Manage a moderate size mouse colony, including genotyping and performing mouse surgery (stereotactic injection of viral expression vectors)
• Perform molecular biology procedures including plasmid DNA preparation, PCR, subcloning, biochemical assays, bacterial expression of fluorescent proteins, and fluorescence analysis
• Perform a small amount of tissue culture (cell line maintenance and transfection)
• Opportunity to do semi-independent projects on fluorescent biosensor development and/or biosensor imaging

Benefits

• Generous paid time off including parental leave
• Medical, dental, and vision health insurance coverage starting on day one
• Retirement plans with university contributions
• Wellbeing and mental health resources
• Support for families and caregivers
• Professional development opportunities including tuition assistance and reimbursement
• Commuter benefits, discounts and campus perks

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Associate degree