Research Assistant II

Vanderbilt University Medical Center • Nashville, TN

8d • Onsite

About The Position

A laboratory in the Department of Pathology, Microbiology and Immunology is seeking a Research Assistant II (RAII) to join our team. In this role, you’ll be a key laboratory resource—supporting day-to-day operations while contributing directly to ongoing research projects in a fast-paced, collaborative environment. With moderate supervision, you will set up and conduct experiments, follow established protocols, and ensure accurate documentation and reporting of results (including any unexpected or variant findings). Your work will include: Culturing cells (eukaryotic and prokaryotic), Performing molecular biology techniques such as transformations, DNA preparation, PCR, and sequence analysis, Expressing and purifying proteins, Performing flow cytometry, Preparing common reagents and solutions, Supporting routine maintenance of laboratory equipment, Managing inventory of reagents and supplies, Assisting with other laboratory and research activities as needed.

Requirements

• Bachelor’s degree (or equivalent experience)
• 2 years of experience in molecular biology

Nice To Haves

• Experience with plasmid preparation, cloning, DNA mutagenesis, and cultured mammalian cells

Responsibilities

• Under moderate supervision, conduct and evaluate experiments and assays
• Understand and follow experimental protocols (may include animal handling, tissue culture, etc.)
• Record, compile, interpret, and communicate data/results
• Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogens)
• Perform calculations involving molarity, % solutions, and serial dilutions
• Prepare, operate, and troubleshoot equipment in experimental procedures
• Read and follow all equipment manuals
• Maintain and repair equipment according to manufacturer and University guidelines
• Ensure Laboratory is prepared for experiments
• Maintain inventories
• Maintain sterile conditions as required
• Prepare reagents
• Demonstrate an understanding of acceptable procedures and techniques
• Recognize problems and suggest solutions
• Assist in modifications as directed
• Provide appropriate written documentation of experiments
• Prepare preliminary research progress reports
• Summarize test results appropriately
• Culturing cells (eukaryotic and prokaryotic)
• Performing molecular biology techniques such as transformations, DNA preparation, PCR, and sequence analysis
• Expressing and purifying proteins
• Performing flow cytometry
• Preparing common reagents and solutions
• Supporting routine maintenance of laboratory equipment
• Managing inventory of reagents and supplies
• Assisting with other laboratory and research activities as needed

Benefits

• health
• disability
• retirement
• wellness offerings

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level