Research Assistant I - Griffin Lab

University of FloridaPalm Beach, FL

About The Position

The Griffin Lab is seeking a motivated research assistant to support studies on nuclear receptor signaling and innate/adaptive immune responses. The successful candidate will perform primary cell culture, characterize immune cell responses, and analyze target gene expression changes using biochemical, molecular and cellular biology techniques.

Requirements

  • Hands-on experience with primary cell culture and aseptic technique.
  • Experience with RT-qPCR and basic molecular biology methods including plasmid transfection.
  • Strong organizational skills and attention to detail.

Nice To Haves

  • MS in immunology, cell biology, molecular biology or related field.
  • Experience with flow cytometry.
  • Experience with immune cell isolation and functional assays.
  • Familiarity with RNA-seq sample prep or bioinformatics basics.
  • Prior experience in an academic research setting.

Responsibilities

  • Isolate, culture, and maintain primary cells.
  • Perform immune stimulation assays.
  • Quantify target gene expression changes via RT-qPCR and assist with RNA-seq and CUT&RUN sample preparation.
  • Maintain cell lines and perform routine molecular biology (RNA/ DNA extraction, cloning, western blot).
  • Maintain detailed lab notebooks and assist with data analysis/ figure preparation.
  • Manage lab inventory, reagent ordering, and equipment maintenance.
  • Assist with IACUC-related animal procedures (if applicable).
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