Research Assistant I - Laboratory of Dr. Ron Evans

Salk Institute Office of Technology Development•San Diego, CA

4d•$18 - $21•Onsite

About The Position

The Research Assistant I will support NIH R01-funded research focused on human induced pluripotent stem cell (iPSC) differentiation and beta cell transplantation for the development of novel cell replacement therapies for diabetes. Working under the direction of the Principal Investigator and in collaboration with postdoctoral fellows, staff scientists, and research collaborators, the incumbent will assist with maintaining human iPSC cultures, performing multistage pancreatic and beta cell differentiation protocols, characterizing differentiated cells, and supporting transplantation studies in mouse models of diabetes. The successful candidate will be responsible for following established laboratory protocols, maintaining accurate and detailed experimental records, and ensuring consistent application of aseptic techniques and quality standards. This position requires strong organizational skills, attention to detail, and the ability to execute complex, time-sensitive procedures with accuracy and reproducibility. The Research Assistant I will have the opportunity to develop technical expertise in stem cell biology, molecular and cellular biology techniques, genome engineering, and small-animal research while contributing to a collaborative and multidisciplinary research environment.

Requirements

No professional experience required.
Education: Bachelor's degree in Biological Sciences or related scientific discipline required. A minimum of 2 years of experience in a scientific role is required for consideration of a Bachelor's degree in a different discipline.
Computer skills required: Microsoft Office
Prior experience or internships in a laboratory setting.
Prior experience with histology, which may be gained from coursework and/or work experience.
Basic knowledge of laboratory procedures and safety practices.
Familiarity with specific laboratory techniques or equipment relevant to the Institute’s operations.
Knowledge of relevant regulations and compliance standards.
Academic laboratory experience applying basic cell biology, molecular biology, biochemistry, or tissue culture principles.
Experience maintaining organized laboratory records and following written protocols accurately.

Responsibilities

Maintain, expand, passage, cryopreserve, and recover human iPSC lines using sterile technique.
Prepare reagents and support multistage differentiation of iPSCs into pancreatic progenitors, endocrine progenitors, and beta cell or islet-like organoids.
Monitor cell culture quality, differentiation progress, and maintain accurate experimental records.
Assist with banking, quality control, and characterization of genetically engineered iPSC lines.
Perform DNA, RNA, and protein extraction and assist with molecular and cellular assays, including PCR, RT-qPCR, immunoblotting, ELISA, immunofluorescence, flow cytometry, and microscopy.
Support analysis of beta cell differentiation and function through marker expression, viability assays, insulin/C-peptide measurements, and glucose-stimulated insulin secretion assays.
Maintain accurate sample records, inventories, and experimental data.
Assist with preparation and transplantation of iPSC-derived beta cell clusters or islet-like organoids under approved animal protocols.
Assist with animal handling, monitoring, dosing, blood glucose measurements, and sample collection following appropriate training.
Support tissue collection, processing, and analysis while maintaining required animal and experimental records.
Prepare media, buffers, solutions, and reagents; maintain laboratory supplies, equipment, and inventory.
Assist with ordering, receiving, labeling, and storage of laboratory materials.
Follow laboratory safety procedures and participate in meetings, data discussions, and troubleshooting activities.
Performs other related duties as assigned by management.