Research Assistant I NEUROLOGY krl
About The Position
The candidate will work independently under the supervision of Joanna Korecka, PhD, and a senior researcher in the Korecka lab (postdoctoral fellow). The role will range from molecular biology-focused bench work, mouse tissue processing, to development and optimization of assays for viability/toxicity in iPSC (induced pluripotent stem cells) derived neurons. These assays will include techniques such as flow cytometry, longitudinal imaging of neurons, plate-based live cell and calorimetric assays and western blotting. The candidate is expected to be diligent, highly organized, and collegial. Position provides assistance to the Principal Investigator and Study Team in coordinating research activities that may include conducting a variety of routine experiments with established methodologies, data collection and management, and other research activities as assigned.
Requirements
- Bachelor's Degree Science required
- Excellent oral and written communication skills.
- Strong interpersonal skills are required for working in a highly diverse lab.
- Excellent organizational skills and ability to prioritize a variety of tasks.
- Excellent data management skills and attention to detail.
- Computer literacy, including database tools.
- Ability to follow directions and exhibit professionalism.
Nice To Haves
- Good presentation skills are a plus.
Responsibilities
- Maintenance, passaging, cataloguing, and culturing of iPSC cells.
- Differentiation iPSCs to neurons and glia and aging of these cultures.
- Maintenance of Attune flow cytometry and ordering the related reagents.
- Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope.
- Maintenance of HEK293 cells, generation of stable cell lines.
- Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps.
- Analysis of data from western blot/ microscopy/ flow cytometry.
- Mouse handling and pre and post-op care.
- Mouse tissue processing, immunohistochemistry, and confocal imaging.
- Live cell calcium and MEA (multielectrode array) computational data analysis.
- The candidate will have excellent lab note-keeping habits, mostly digital(LabArchieves) and will generate Standard Operating Procedures (SOPs) for the lab.
- The candidate will have excellent organizational skills, labeling of experiments, file management.
- The candidate will report daily to the immediate supervisor and weekly to the Principal investigator.
- The candidate will interact with junior and senior members of the lab to troubleshoot experiments.
- The candidate will share lab duties with other technicians.
- Responsible for data collection, management, manipulation, analysis, and reporting.
- Study cohort management.
- Outreach communication with subjects, conducting mailings, follow-up on mailings, and obtaining necessary documents.
- Provides assistance on work related to epidemiological studies and other population-based research.
- Performs other duties as assigned.
- Complies with all policies and standards.
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Number of Employees
5,001-10,000 employees
