Research Assistant 3 - ON SITE
About The Position
The Georgiev laboratory at Vanderbilt University Medical Center (VUMC) is seeking a qualified Research Assistant III (RAIII). This role is crucial for making essential contributions to research projects by performing a variety of laboratory tasks. The RAIII will be responsible for culturing cells (eukaryotic and prokaryotic), conducting molecular biology techniques such as transformations, DNA preparation, PCR, and sequence analysis, expressing and purifying proteins, performing phage display, flow cytometry, and ELISA assays. Additionally, the position involves preparing common reagents, maintaining lab equipment, and managing inventory of reagents and supplies. The successful candidate will work under minimal supervision, set up and conduct experiments, follow protocols, record data accurately, and report results. This role also supports other laboratory and research functions. Exceptional organizational, communication (verbal and written), and attention to detail skills are required, along with the ability to be a collaborative team member. Previous experience with plasmid preparation, cloning, DNA mutagenesis, phage display library construction and screening, ELISA, and culturing/transfecting mammalian cells is preferred. The position is on-site at Vanderbilt Medical Center North in Nashville, TN, with traditional daytime business hours, Monday-Friday.
Requirements
- Bachelor's Degree (or equivalent experience)
- Minimum of 2 years of relevant experience
- Exceptional organizational skills
- Exceptional verbal communication skills
- Exceptional written communication skills
- Attention to detail
- Ability to function as part of a dynamic research team
Nice To Haves
- Plasmid preparation
- Cloning
- DNA mutagenesis
- Phage display library construction and screening
- ELISA
- Culturing and transfecting mammalian cells
Responsibilities
- Plan, conduct and evaluate experiments and assays of varying complexity under minimal supervision.
- Understand and follow experimental protocols, which may include animal handling and tissue culture.
- Record, compile, interpret, and communicate data/results.
- Follow University safety protocols, which may include working with combustibles, radioactivity, toxic materials, and/or carcinogens.
- Perform calculations involving molarity, % solutions, and serial dilutions.
- Prepare, operate, and troubleshoot equipment in experimental procedures.
- Recommend equipment and supplies needed for research projects.
- Read and follow all equipment manuals.
- Maintain and repair equipment according to manufacturer and University guidelines.
- Ensure the laboratory is prepared for experiments.
- Maintain inventories.
- Maintain sterile conditions as required.
- Prepare reagents.
- Assist PI in modifying procedures and techniques.
- Recognize problems and suggest solutions, assisting in modifications.
- Recommend improvements to established techniques and/or protocols.
- Maintain personal knowledge of scientific trends through literature reviews, attendance at meetings, conferences, and seminars.
- Provide appropriate written/oral documentation of experiments.
- Prepare research progress reports.
- Analyze and summarize test results.
- Prepare and orally present data as required.
- Assist in the effective operation of the laboratory.
- Train other employees.
- Supervise projects and/or personnel.
Benefits
- Health offerings
- Disability offerings
- Retirement offerings
- Wellness offerings
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
