Postdoctoral Fellow – Lipotoxicity and Liver Inflammation Laboratory

Mayo Clinic • Rochester, MN

16h

About The Position

Key Responsibilities Conduct independent and collaborative research to investigate mechanisms of liver injury, inflammation, and vascular dysfunction in MASH. Design and execute experiments using in vitro and in vivo models of lipotoxic liver disease. Perform advanced molecular and cellular biology techniques , including but not limited to plasmid cloning, gene expression analysis, protein assays, and cell signaling studies. Isolate and characterize primary liver cells , including intrahepatic leukocytes, hepatocytes and liver sinusoidal endothelial cells. Manage and perform rodent handling and interventions , including diet-induced MASH models and genetic or pharmacologic manipulations. Apply flow cytometry, immunohistochemistry, and immunofluorescence techniques to assess cell populations and pathological changes. Perform basic bioinformatic analysis (e.g., RNA-seq) and interpret complex datasets. Participate in manuscript preparation, presentations, and grant writing. What You Will Gain Training in cutting-edge methodologies relevant to liver disease and immunometabolism. Collaborative environment at a leading academic medical research center. Opportunity to contribute to high-impact publications and translational research advancing therapeutic strategies for MASH. Qualifications Required Qualifications Ph.D. in Physiology and biomedical engineering, biochemistry and Molecular Biology, Immunology, Vascular Biology, or related biomedical discipline. Demonstrated expertise in liver pathobiology and basic molecular and mechanistic techniques . Hands-on experience with animal handling , including approval and conduct of rodent studies. Proven ability with plasmid cloning , gene manipulation, and cell isolation procedures. Competence in basic bioinformatic data analysis (e.g., differential gene expression, pathway analysis). Solid background in immunology , particularly as related to inflammation and cellular crosstalk. Familiarity with vascular biology or endothelial cell function in organ pathology is highly desirable. Preferred Skills Use of omics approaches to understand tissue zonation and cell interactions. Experience in studying intercellular signaling . Strong written and oral communication skills suitable for presentations and publications.

Requirements

• Ph.D. in Physiology and biomedical engineering, biochemistry and Molecular Biology, Immunology, Vascular Biology, or related biomedical discipline.
• Demonstrated expertise in liver pathobiology and basic molecular and mechanistic techniques .
• Hands-on experience with animal handling , including approval and conduct of rodent studies.
• Proven ability with plasmid cloning , gene manipulation, and cell isolation procedures.
• Competence in basic bioinformatic data analysis (e.g., differential gene expression, pathway analysis).
• Solid background in immunology , particularly as related to inflammation and cellular crosstalk.
• Familiarity with vascular biology or endothelial cell function in organ pathology is highly desirable.

Nice To Haves

• Use of omics approaches to understand tissue zonation and cell interactions.
• Experience in studying intercellular signaling .
• Strong written and oral communication skills suitable for presentations and publications.

Responsibilities

• Conduct independent and collaborative research to investigate mechanisms of liver injury, inflammation, and vascular dysfunction in MASH.
• Design and execute experiments using in vitro and in vivo models of lipotoxic liver disease.
• Perform advanced molecular and cellular biology techniques , including but not limited to plasmid cloning, gene expression analysis, protein assays, and cell signaling studies.
• Isolate and characterize primary liver cells , including intrahepatic leukocytes, hepatocytes and liver sinusoidal endothelial cells.
• Manage and perform rodent handling and interventions , including diet-induced MASH models and genetic or pharmacologic manipulations.
• Apply flow cytometry, immunohistochemistry, and immunofluorescence techniques to assess cell populations and pathological changes.
• Perform basic bioinformatic analysis (e.g., RNA-seq) and interpret complex datasets.
• Participate in manuscript preparation, presentations, and grant writing.

What This Job Offers

• Job Type: Full-time
• Education Level: Ph.D. or professional degree