Post-Doctoral Research Associate: Department of Microbiology - UTK
About The Position
The Clasen lab in the Department of Microbiology at UTK is seeking a post-doctoral researcher with expertise in structural biology. The lab studies the relationship between the host immune system and the commensal microbes that colonize the intestinal tract. We seek to understand how host immune responses impact microbial behavior. To address this, we employ a combination of techniques in molecular biology and immunology, next generation sequencing, and in vitro and in vivo models. This position contributes to the daily operations of the laboratory by ordering and maintaining lab supplies, organizing and managing laboratory space and databases, and supporting new lab members and experimental projects. The role requires performing and troubleshooting a variety of experiments while maintaining detailed laboratory records. Experimental techniques may include protein analysis such as expression and purification, cryo-EM, ELISA, western blotting, and immunoprecipitation; cell analysis including cell sorting, separation, and flow cytometry; bacterial culturing under both aerobic and anaerobic conditions; molecular biology techniques such as cloning, DNA/RNA extraction, and PCR; microscopy; and 2D and 3D cell culture. Additional responsibilities may include mouse organ dissection and preparation of cells and tissues for downstream assays. The position also involves working with animals and maintaining mouse colonies by serving as the primary health contact for animals, managing detailed colony and breeding records, and performing mouse tagging, weaning, and genotyping.
Requirements
- Expertise in structural biology
- Experience with molecular biology and immunology techniques
- Experience with next generation sequencing
- Experience with in vitro and in vivo models
- Experience with protein analysis (expression, purification, cryo-EM, ELISA, western blotting, immunoprecipitation)
- Experience with cell analysis (cell sorting, separation, flow cytometry)
- Experience with bacterial culturing (aerobic and anaerobic)
- Experience with molecular biology techniques (cloning, DNA/RNA extraction, PCR)
- Experience with microscopy
- Experience with 2D and 3D cell culture
- Experience with mouse organ dissection
- Experience preparing cells and tissues for downstream assays
- Experience working with animals
- Experience maintaining mouse colonies
- Experience managing detailed colony and breeding records
- Experience performing mouse tagging, weaning, and genotyping
Responsibilities
- Ordering and maintaining lab supplies
- Organizing and managing laboratory space and databases
- Supporting new lab members and experimental projects
- Performing and troubleshooting a variety of experiments
- Maintaining detailed laboratory records
- Protein analysis such as expression and purification, cryo-EM, ELISA, western blotting, and immunoprecipitation
- Cell analysis including cell sorting, separation, and flow cytometry
- Bacterial culturing under both aerobic and anaerobic conditions
- Molecular biology techniques such as cloning, DNA/RNA extraction, and PCR
- Microscopy
- 2D and 3D cell culture
- Mouse organ dissection and preparation of cells and tissues for downstream assays
- Working with animals and maintaining mouse colonies
- Serving as the primary health contact for animals
- Managing detailed colony and breeding records
- Performing mouse tagging, weaning, and genotyping
Benefits
- Career development and coaching
- Continued education
- Extensive list of development and training possibilities
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Education Level
Ph.D. or professional degree
