OPS Laboratory Assistant
About The Position
This position is housed in the Department of Physical Therapy in the College of Public Health and Health Professions and the Biological Sciences Building. The incumbent will assist the Principal Investigator, Dr. Russell Hepple, in investigations concerning the impact of aging on skeletal muscle. Tasks include preparation of solutions and reagents used in experiments, rodent tissue harvest, preparation of muscle bundles, preparation of isolated mitochondria, and preparation of single muscle fibers. In addition, the incumbent will maintain equipment (pH meter, respirometers, fluorometer, cryostat, microscope, -80C freezer), order lab supplies and maintain records of inventory.
Requirements
- Bachelor’s degree in biology or a closely related life science field.
Nice To Haves
- 1-2 years prior lab experience
- Experience with molecular biology techniques (immunolabeling of tissue, qPCR, Western blotting, immunoprecipitation)
Responsibilities
- Prepare lab reagents
- Perform tissue harvest from experimental animals
- Prepare muscle bundles and isolated mitochondria for mitochondrial function (respirometry, ROS emission, and Calcium Retention Capacity (CRC) assays)
- General laboratory maintenance, including maintaining adequate lab supplies (keeping inventories, ordering), troubleshooting laboratory equipment, safety compliance, etc.
- Ensure cleanliness and functionality of surgical suite
- Prepare and autoclave surgical tools
- Keep records of mouse colonies and liaise with ACS staff for medical treatments
- Administer tamoxifen injections in transgenic mice
- Basic repairs and installations of equipment, calibration and required maintenance of respirometers, fluorometer and pH meter
- Other duties as necessary
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What This Job Offers
Job Type
Part-time
Career Level
Entry Level
