Laboratory Research Technician IV

University of Pittsburgh • Pittsburgh, PA

9d • Onsite

About The Position

The University of Pittsburgh School of Medicine seeks a Laboratory Research Technician IV to work in Pittsburgh, PA. Duties include designing/performing: (i) isolation, activation, and expansion of T cells (ii) cryopreservation, sub-culturing, and revival of various cell lines and primary cells; (iii) cell-based assays including cytotoxicity, cell viability, and cell proliferation assays; (iv) immunofluorescence imaging using confocal microscopy and single-cell time-lapse imaging; (v) isolation and handling of PBMCs from human whole blood; (vi) multicolor flow cytometry, SDS-PAGE, western blot, multi-mode reader-based assays and various biochemical assays; and (vii) data analysis using Graphpad Prism, FlowJo, Excel and Image-J.

Requirements

• Master’s degree (or foreign equivalent degree) in Biology, Biotechnology, or a related field
• One (1) year of experience in a related position
• Experience working with Cryopreservation, sub-culturing and revival of various cell lines, mammalian cell lines and primary cells
• Experience with In-vitro assays such as cytotoxicity, cell viability, cell proliferation
• Experience with Live cell time-lapse imaging for cell viability assays, spheroids, immune cell killing, co-culture assays and image analysis
• Experience with Isolation of and assays using Peripheral Blood Mononuclear Cells (PBMCs) from human blood
• Experience with isolation and expansion of naïve T cells, CD4 T cells, CD8 T cells, and regulatory T cells
• Experience with Activation and expansion of T cells using Dynabeads and plate coated antigen
• Experience with Screening of peptide pools, cytokine/chemokine profiling using LUMINEX, and direct, indirect, Quantikine, and sandwich ELISA assays
• Experience with Seed, treat, fix, permeabilize, block and antibody staining/addition of cell lines and primary immune cells for multicolor flow cytometry of surface and intracellular markers using appropriate compensation and FMO controls for different multi-point in-vitro assays
• Experience with Analysis of the flow cytometry data for receptor quantification and drug screening using Flow-Jo Software
• Experience with Preparing cells for molecular biology techniques such as SDS-PAGE, western Blot, protein/antibody quantification using Bradford/BCA assay and other multi-mode reader-based assays, bacterial transformation and cloning
• Experience with data analysis using Graphpad Prism and Excel
• Experience with Image analysis using Image-J

Responsibilities

• Isolation, activation, and expansion of T cells
• Cryopreservation, sub-culturing, and revival of various cell lines and primary cells
• Cell-based assays including cytotoxicity, cell viability, and cell proliferation assays
• Immunofluorescence imaging using confocal microscopy and single-cell time-lapse imaging
• Isolation and handling of PBMCs from human whole blood
• Multicolor flow cytometry, SDS-PAGE, western blot, multi-mode reader-based assays and various biochemical assays
• Data analysis using Graphpad Prism, FlowJo, Excel and Image-J

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level