Laboratory and Research Specialist II
About The Position
The Laboratory and Research Specialist will conduct growth experiments with pure cultures of strictly anaerobic, hyperthermophilic and mesophilic methanogenic archaea, employing serum tubes and serum bottles, and analyze these cultures employing UV-visible spectroscopy, light microscopy and protein assays. The Laboratory and Research Specialist will formulate and prepare appropriate growth media and cultivate strictly anaerobic, hyperthermophilic and mesophilic methanogenic archaea in these media (5-100 mL) and follow their growth by cell count as determined by use of a phase contrast microscope fitted with a Petroff-Hauser counter and optical density. The Specialist will perform heterologous protein expression experiments with recombinant hyperthermophilic and mesophilic methanogenic archaea and E. coli, estimate the levels of heterologous proteins expressed via SDS-PAGE and western blotting and purify these proteins to apparent homogeneity under aerobic and anaerobic conditions via affinity chromatography. Experiences in using an anaerobic chamber, working with strictly anaerobic methanogenic archaea are must, estimation of protein, and use of a spectrophotometer are essential.
Requirements
- B.S. Biochemistry or Microbiology or equivalent relevant experience and training.
- Research experience in the cultivation of strictly anaerobic methanogenic archaea in liquid media with gaseous substrates.
- Experience using an anaerobic chamber.
- Experience purifying heterologously expressed proteins to apparent homogeneity.
- Experience analyzing proteins via SDS-PAGE, western blotting and protein assay.
- Demonstrated experience with data processing using Microsoft Excel.
- Experience writing reports.
Nice To Haves
- Experience in performing spectrophotometric assays for enzymatic activity.
Responsibilities
- Conduct growth experiments with pure cultures of strictly anaerobic, hyperthermophilic and mesophilic methanogenic archaea.
- Analyze cultures employing UV-visible spectroscopy, light microscopy and protein assays.
- Formulate and prepare appropriate growth media.
- Cultivate strictly anaerobic, hyperthermophilic and mesophilic methanogenic archaea in growth media.
- Follow growth by cell count using a phase contrast microscope fitted with a Petroff-Hauser counter and optical density.
- Perform heterologous protein expression experiments with recombinant hyperthermophilic and mesophilic methanogenic archaea and E. coli.
- Estimate levels of heterologous proteins expressed via SDS-PAGE and western blotting.
- Purify proteins to apparent homogeneity under aerobic and anaerobic conditions via affinity chromatography.
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What This Job Offers
Job Type
Part-time
Career Level
Mid Level
Education Level
Associate degree
