Core Sr Research Assistant (Temporary)

Staff Career•La Jolla, CA

6d•Onsite

About The Position

The Cell Technologies and Engineering Core Research Assistant will work on problems of diverse scope related to the use of human stem cells and derivation of somatic cell populations for disease modeling applications. This position will primarily support human cell modeling needs associated with multiple research centers on campus, including centers focused on novel cell modeling approaches for aging and Alzheimer’s disease. The Senior Research Assistant will exercise judgement within generally defined practices and policies in selecting methods and techniques for obtaining solutions. Normally receives general instructions on routine work and new assignments. Work is performed under general supervision. The expected duration for this position is up to 3 months; however, employment is at-will, and the duration may change based on business needs.

Requirements

Bachelor's Degree (BA) in relevant field
2 years of relevant experience, or Equivalent combination of education/experience, or one to two years of related experience and/or training, or equivalent combination of education and experience.
Experience in human cell culture research environment
Experience with standard cell and molecular biology assays
Experience in CRISPR design and methods
Experience in growing a variety of cell lines from a single cell
Experience genotyping and troubleshooting
Basic light and fluorescence microscopy
Computer skills required: Microsoft Office, Microscopy imaging and analysis software

Responsibilities

Cultures various human cells lines, such as primary dermal fibroblasts, embryonic stem cell, induced pluripotent stem cells, as well as other somatic and immortalized human cell populations.
Performs various molecular biology techniques: DNA/RNA extraction, molecular cloning, restriction enzyme digestion, ligation, amplification and purification of plasmid DNA vectors, PCR, gel electrophoresis, recombinant DNA techniques and vector design.
Performs CRISPR gene targeting using various formats (ie, RNP vs plasmid delivery), variations (Prime Editing vs original) and goals. Will contribute to design of guides, delivery method, validation, and genotyping assays.
Maintains accurate records of all cells entering and leaving storage, maintains detailed lab notebooks accurately recording all manipulations and treatments to cells and deviations from protocols.
Performs instrument maintenance including regular cleaning and calibration.
Responsible for supply inventory management.
Assist director in cryobanking inventory updates and transition to new system.
Delivers customer service and training.
Performs other related duties as assigned by management.