Chemist II - Oligonucleotide

Bureau Veritas•Indianapolis, IN

9d•$28 - $31•Onsite

About The Position

The Chemist II is responsible for assisting with synthesis and downstream processing of oligo synthesis using various analytical techniques. For Oligosynthesis, the Dr. Oligo and MerMade 192 oligosynthesizers are used. For purification and analysis AKTA, LC-MS, UPLC, LTQ, QDA analytical instruments are used. For downstream automation a Lynx liquid handler will be used. This person will assist with training new staff, work on multiple projects simultaneously and stay current with new modifications of oligonucleotides.

Requirements

Experience, troubleshooting, and optimizing synthetic protocols on various synthesizers
Experience with Automation instruments such as Tecan, Lynx, Dispendix, Hamilton, Beckman, etc.
Strong laboratory skills and experience with analytical instrumentation such as AKTA, Agilent LC-MS and UPLC, Vanquish LTQ, Water’s QDA, etc.
Strong organizational skills and ability to prepare written technical reports
Strong oral communication skills in presenting facts and recommendations
Confident, execution-focused, and team oriented with a desire to thrive in a fast-paced growing environment
Written and verbal communication skills including the ability to communicate effectively in a group setting, with colleagues and clients
Ability to perform multiple tasks
Ability to organize and prioritize workload to meet or exceed deadlines
Individual and Team work ethic
Proficiency with Windows, Word & Excel
Attention to detail
Positive attitude, self-motivated, high level of engagement
Champions and adheres to company values including team engagement
Bachelor's Degree in Chemistry, Biochemistry, or closely related field
Minimum of one (1) year of experience with oligonucleotide chemistry or plate based automation development
An equivalent combination of education and experience may be accepted in lieu of above

Responsibilities

Independently perform synthesis and downstream processing of oligo synthesis: cleavage, deprotection, purification, desalting and annealing
Bioconjugation of single strand oligonucleotide
Use of general laboratory equipment including but not limited to automation instruments, pipettors, centrifuges, incubators, biological safety cabinets and balances
Instrumentation for methods and techniques described include various synthesizers, LC-MS, UPLC, LTQ, QDA, Nanodrop, etc.
Familiarity with various statistical analysis tools and able to solve technical problems and make recommendations for improvement
Work closely with members of the RNAi plate synthesis team to understand and validate requirements for synthesis and downstream processing
Review literature to stay current on new methods for oligonucleotides and provide technical solutions to clients and management
Lab experience with solution preparation, separation, quantification, synthesis methodologies for DNA/RNA, proteins, oligonucleotides, etc.
Software includes instrument-specific for capturing and analyzing data, Microsoft Office (Word, Excel, PowerPoint, Outlook, Teams/Sharepoint, etc), LIMS, electronic laboratory notebooks, etc.
Detailed record keeping of daily experiments in ELNs
General Lab Maintenance
Train new staff members
Identify, troubleshoot, and suggest technical solutions to clients and management
Follow the guidelines set forth with clients and Company Quality, Health, Safety, Security and Environmental policies and procedures.
Comply with clients’ and Company management systems in accordance with appropriate regulatory agencies.
Perform other essential tasks projects and responsibilities as required.
Other duties as defined by the Manager, department needs and workload