Cell Culture Technician

University of British Columbia • Vancouver, BC

4d • CA$29 - CA$34 • Onsite

About The Position

The Vallance laboratory is part of the Childhood Diseases Research Theme of the BC Children's Hospital Research Institute and is a world leader in studying inflammatory bowel disease (IBD) and enteric bacterial infections. The Vallance lab focuses on nutrition - bacteria - host interactions at the intestinal mucosal interface. We are looking for a motivated and talented individual to join our team as a Research Technician. The successful candidate will work on a cutting-edge project using pre-clinical organoid models to develop novel therapies for Inflammatory Bowel Disease. Specifically, the successful candidate will receive patient intestinal biopsies and generate intestinal organoids. These patient-derived intestinal organoids will be cultured and maintained, as well as studied using routine and standardized assays. The successful candidate will assist senior staff and trainees in growing and analyzing organoids, using modern techniques, performed under minimal supervision. The research technician role requires extensive laboratory and cell culture experience. While organoid experience would be an asset, those without this experience will be trained in the required procedures and techniques. It is expected that the successful candidate will master specific technical skills underlying primary cell culture technique and be sufficiently skilled to independently perform such work and instruct others as well. As well the role will involve the incumbent working closely with an interdisciplinary team to tackle cutting-edge challenges in organoid work.

Requirements

• Completion of a relevant technical program or a university degree in a relevant discipline and a minimum three years of related experience or an equivalent combination of education and experience.
• Expertise in general cell culture using cell lines or primary human cells.
• Minimum of 3 years related laboratory experience or the equivalent combination of education and experience.
• Prior experience working in a laboratory environment is mandatory
• Ability to exercise sound judgement, responsibility, and maintain confidentiality.
• Ability to communicate effectively, both verbally and in writing.
• Ability to work both independently and within a collaborative team environment.
• Demonstrated ability to meet short- and long-term project deadlines.
• Strong time management and organization skills.

Nice To Haves

• Organoid experience would be an asset

Responsibilities

• Process intestinal biopsies for organoid isolation derived from patient samples and maintain collection records
• Oversee the daily operation of the lab’s organoid maintenance, including culturing and passaging of intestinal organoid cultures.
• Prepare organoid media and performing routine testing to identify possible contamination following the lab’s SOPs.
• Perform routine standardized assays to evaluate organoid quality and function
• Maintain inventory of organoid lines
• Plan and perform experiments; troubleshoot problems; collect, record, analyse and interpret experimental results with senior staff
• Maintain a detailed lab notebook
• Once sufficiently experienced, provide training to undergraduate and graduate students, as well as new and lower level technicians in the use of equipment, techniques and procedures.
• Set up, test, operate and maintain common laboratory equipment and laboratory reagents.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Associate degree