Biomedical Technician

Cedars-Sinai•Beverly Hills, CA

22h•Onsite

About The Position

The Applied Genomics, Computation & Translational Core (AGCT Core) is seeking a Biomedical Technician to join their team. This role contributes to the laboratory sciences team, working independently under the direct supervision of a team lead. The technician will perform laboratory benchwork strictly following Standard Operating Procedures (SOPs) with accurate documentation and a theoretical understanding of the assays used. Responsibilities include performing quality control on RNA and DNA samples, understanding sample quality nuances, and contributing to project success through effective communication, documentation, and attention to detail. The role involves working with state-of-the-art technologies and instruments, including performing next-generation sequencing library preparation and sequencing.

Requirements

Strict adherence to Standard Operating Procedures (SOPs).
Accurate documentation.
Theoretical understanding of assays utilized.
Quality control on RNA and DNA samples.
Understanding of sample quality and qualifications.
Good oral and written communication.
Attentiveness to detail.
Experience with state-of-the-art technologies and instruments.
Performing next generation sequencing library preparation and sequencing.
Experience with Illumina short-read sequencing and/or Oxford Nanopore Technologies long-read sequencing of NGS libraries.
Experience with DNA/RNA QC via fluorescent quantification and automated electrophoresis.
Operation of genomics equipment including NGS sequencers, automated liquid handlers, and tissue imagers, as well as standard laboratory equipment such as thermal cyclers, centrifuges, and qPCR machines.
Proficiency in mathematical calculations, including fold dilution, normalization, and conversions.
Ability to understand, analyze, and interpret DNA/RNA/NGS library/sequencing QC results.
Ability to liaise with researchers and investigators to provide quality customer service.
Ability to handle multiple demands and manage complex and competing priorities.
Ability to apply advanced knowledge of science/learning/specialized intellectual instructions to analyze, interpret, or make deductions.
Ability to provide and/or support a level of work excellence and accuracy; recognize and address flaws or errors.
Regular oral and written communication with team members and team lead.
Accurate and detailed electronic documentation of work performed and results obtained.
Ability to work independently as part of a team under direct supervision.
Observation and compliance with all safety standards and procedures.

Nice To Haves

Experience with 10x Genomics (Chromium X, Xenium Analyzer, Visium CytAssist), Parse Biosciences, Scale Biosciences, Singular Genomics G4X, and Bruker CosMX SMI.
Experience with Illumina NovaSeq X Plus.
Expertise in long-read sequencing via Oxford Nanopore Technologies (PromethION 24, MinION).
Expertise in high-throughput short-read sequencing on the Illumina NovaSeq X Plus.
Experience with bulk cell RNA-Seq, WGS/WES, ATAC-Seq, and metagenomics.
Experience with standard pipelines for primary data processing (read alignment, quality control, expression quantification, variant calling, peak calling).
Experience with downstream analysis packages.
Experience developing sophisticated or custom computational solutions.
Experience with advanced analysis for spatial transcriptomics data.
Experience with results interpretation, generation of publication-ready figures, and drafting manuscript methodology.

Responsibilities

Performs a variety of project-specific process development tasks for multiple clients following standard operating procedures (SOPs).
Maintains good documentation in laboratory notebooks, data record/transfer, SOPs, and batch records.
Drafts standard operating procedures and maintains computer database.
Verifies samples received, logs samples into systems, and may transport to appropriate environment within the facility for storage following SOPs.
Performs lab maintenance duties, including glassware cleaning and sterilization.
Maintains lab equipment and related records.
Prepares cGMP production room, maintains material inventories, and places orders for equipment and supplies.
Assists in the operation of specialized equipment and machinery.
Ensures all activities comply with regulatory guidelines and safety standards, as appropriate.
Construction of next generation sequencing libraries for bulk cell, single cell, and/or spatial omics using commercially available reagent kits.
Illumina short-read sequencing and/or Oxford Nanopore Technologies long-read sequencing of NGS libraries.
DNA/RNA QC via fluorescent quantification and automated electrophoresis.
Operation of state-of-the-art genomics equipment including NGS sequencers, automated liquid handlers, and tissue imagers, as well as standard laboratory equipment such as thermal cyclers, centrifuges, and qPCR machines.
Mathematical calculations, including fold dilution, normalization, and conversions.
Understand, analyze, and interpret DNA/RNA/NGS library/sequencing QC results.
Liaise with post-doctoral, doctoral, and staff researchers, clinical investigators, and principal investigators to provide quality customer service.
Handle multiple demands and/or manage complex and competing priorities, pivoting as needed based on current needs in a dynamic environment.
Apply advanced knowledge of science/learning/specialized intellectual instructions to analyze, interpret, or make deductions from varying facts or circumstances.
Provide and/or support a level of work excellence and accuracy; recognize and address flaws or errors that others may overlook.
Regular oral and written communication with team members and team lead.
Accurate and detailed electronic documentation of work performed and results obtained.
Work independently as part of a team under direct supervision of a team lead.
Observe and comply with all safety standards and procedures.