Biomedical Technician - Genomics Core

Cedars-Sinai•West Hollywood, CA

9d•$30 - $47•Onsite

About The Position

The Applied Genomics, Computation & Translational Core (AGCT) is seeking a Biomedical Technician to join their team. This role involves performing laboratory benchwork strictly according to Standard Operating Procedures with accurate documentation and a theoretical understanding of assays utilized. The technician will perform quality control on RNA and DNA samples, understanding the nuances of sample quality and qualifications. They will contribute to project success through good oral and written communication, documentation, and attentiveness to detail, working with state-of-the-art technologies and instruments, performing next generation sequencing library preparation and sequencing. The AGCT Core is an automated, high-throughput genomics facility equipped with the latest next-generation sequencing technologies, specializing in single-cell and spatial omics, and offering comprehensive support from sample preparation to diverse sequencing applications and bioinformatics analysis.

Requirements

Experience in single-cell and spatial assay technologies
Familiarity with techniques such as single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, multiplex imaging, and other high-dimensional molecular profiling platforms
Working knowledge of sample preparation workflows, library preparation, quality control metrics, and data generation associated with next-generation sequencing or imaging-based assays
Experience handling complex biological specimens
Ability to maintain rigorous data integrity within high-throughput experimental environments
Performing laboratory benchwork strictly according to Standard Operating Procedures
Accurate documentation
Theoretical understanding of assays utilized
Perform quality control on RNA and DNA samples
Understanding the nuances of sample quality and qualifications
Good oral and written communication
Attentiveness to detail
Performing next generation sequencing library preparation and sequencing
Construction of next generation sequencing libraries for bulk cell, single cell, and/or spatial omics using commercially available reagent kits.
Illumina short-read sequencing and/or Oxford Nanopore Technologies long-read sequencing of NGS libraries.
DNA/RNA QC via fluorescent quantification and automated electrophoresis.
Operation of state-of-the-art genomics equipment including NGS sequencers, automated liquid handlers, and tissue imagers, as well as standard laboratory equipment such as thermal cyclers, centrifuges, and qPCR machines.
Mathematical calculations, including fold dilution, normalization, and conversions.
Understand, analyze, and interpret DNA/RNA/NGS library/sequencing QC results.
Liaise with post-doctoral, doctoral, and staff researchers, clinical investigators, and principal investigators to provide quality customer service.
Handle multiple demands and/or manage complex and competing priorities, pivoting as needed based on current needs in a dynamic environment.
Apply advanced knowledge of science/learning/specialized intellectual instructions to analyze, interpret, or make deductions from varying facts or circumstances.
Provide and/or support a level of work excellence and accuracy; recognize and address flaws or errors that others may overlook.
Regular oral and written communication with team members and team lead.
Accurate and detailed electronic documentation of work performed and results obtained.
Work independently as part of a team under direct supervision of a team lead.
Observe and comply with all safety standards and procedures.

Nice To Haves

Candidates with experience in single-cell and spatial assay technologies are highly desirable.

Responsibilities

Performs a variety of project-specific process development tasks for multiple clients following standard operating procedures (SOPs).
Maintains good documentation in laboratory notebooks, data record/transfer, SOPs, and batch records.
Drafts standard operating procedures and maintains computer database.
Verifies samples received, logs samples into systems, and may transport to appropriate environment within the facility for storage following SOPs.
Performs lab maintenance duties, including glassware cleaning and sterilization.
Maintains lab equipment and related records.
Prepares cGMP production room, maintains material inventories, and places orders for equipment and supplies.
Assists in the operation of specialized equipment and machinery.
Ensures all activities comply with regulatory guidelines and safety standards, as appropriate.
Construction of next generation sequencing libraries for bulk cell, single cell, and/or spatial omics using commercially available reagent kits.
Illumina short-read sequencing and/or Oxford Nanopore Technologies long-read sequencing of NGS libraries.
DNA/RNA QC via fluorescent quantification and automated electrophoresis.
Operation of state-of-the-art genomics equipment including NGS sequencers, automated liquid handlers, and tissue imagers, as well as standard laboratory equipment such as thermal cyclers, centrifuges, and qPCR machines.
Mathematical calculations, including fold dilution, normalization, and conversions.
Understand, analyze, and interpret DNA/RNA/NGS library/sequencing QC results.
Liaise with post-doctoral, doctoral, and staff researchers, clinical investigators, and principal investigators to provide quality customer service.
Handle multiple demands and/or manage complex and competing priorities, pivoting as needed based on current needs in a dynamic environment.
Apply advanced knowledge of science/learning/specialized intellectual instructions to analyze, interpret, or make deductions from varying facts or circumstances.
Provide and/or support a level of work excellence and accuracy; recognize and address flaws or errors that others may overlook.
Regular oral and written communication with team members and team lead.
Accurate and detailed electronic documentation of work performed and results obtained.
Work independently as part of a team under direct supervision of a team lead.
Observe and comply with all safety standards and procedures.