Associate Scientist - Protein Biochemistry (siRNA Therapeutics, Lipid Nanoparticles)

Regeneron Pharmaceuticals • Tarrytown, GA

2d • Hybrid

About The Position

Regeneron's Product Biochemistry group, located in Tarrytown NY, is seeking an Associate Scientistwho will be responsible for providing in-depth characterization of nucleic acid therapeutics and Lipid Nanoparticles (LNP). In this exciting role, you will be expected to participate in technology innovation efforts aimed at enhancing our understanding of oligonucleotides and LNPs in solution and address both pre-clinical and late-stage program needs. A Typical Day in the Role of Associate Scientist Might Look Like: Develop analytical methods and conduct biochemical and biophysical characterization experiments to elucidate quality attributes of gene therapy candidates. Help solve problems in real time. Provide support for basic research efforts. Ability to adapt and/or optimize methods described in the literature. Conduct time-sensitive biochemical and extended characterization of siRNAs to meet regulatory timelines. Provide input on experimental design and suggest method adaptations to address specific needs of a program or attributes of colloidal systems. Additional responsibilities will include method development of advanced technologies that evaluate the purity and/or thermodynamic and hydrodynamic interactions of siRNAs, mRNAs, CRISPR gRNA and LNP-nucleic acid complexes. Support forced degradation studies evaluating environmental stressors and their impact on the stability of siRNA, LNPs and their cargos. Document experiments, results, and findings in electronic laboratory notebook; presents work at group and departmental meetings, contributes to drafting of protocols, method development reports, regulatory submission sections, and peer-reviewed publications. Maintain instruments, troubleshoots instrumental/experimental problems, and order/maintain supplies.

Requirements

• Excellent oral and written communication skills
• Strong initiative and drive to complete challenging tasks with a sense of urgency
• Ability and desire to learn new technologies
• A desire to work both independently and within a collegial and collaborative environment
• BS or MS in Biophysics, Biochemistry, Chemistry, Chemical Engineering, or related field with a minimum of 0-2 years or BS with 2+ years of relevant experience.
• A fundamental understanding of practical biophysical chemistry of macromolecules in solution is essential.
• Basic knowledge of oligonucleotide properties, lipid biochemistry and gene editing therapeutics is important.
• Good laboratory practice skills preparing samples, calculating concentrations, documenting, and maintaining cleanliness are critical.
• Basic practical experience in automated chromatography (FPLC, H/UPLC), spectroscopy (UV-Vis, fluorescence, FTIR and CD) and/or multi-angle/dynamic light scattering (MALS/DLS) of macromolecules is important.

Nice To Haves

• Experience with mass spectrometry (MS), NMR, ELSD/CAD, charge-based methods, and/or advanced imaging skills are a bonus.

Responsibilities

• Develop analytical methods and conduct biochemical and biophysical characterization experiments to elucidate quality attributes of gene therapy candidates.
• Help solve problems in real time.
• Provide support for basic research efforts.
• Ability to adapt and/or optimize methods described in the literature.
• Conduct time-sensitive biochemical and extended characterization of siRNAs to meet regulatory timelines.
• Provide input on experimental design and suggest method adaptations to address specific needs of a program or attributes of colloidal systems.
• Method development of advanced technologies that evaluate the purity and/or thermodynamic and hydrodynamic interactions of siRNAs, mRNAs, CRISPR gRNA and LNP-nucleic acid complexes.
• Support forced degradation studies evaluating environmental stressors and their impact on the stability of siRNA, LNPs and their cargos.
• Document experiments, results, and findings in electronic laboratory notebook; presents work at group and departmental meetings, contributes to drafting of protocols, method development reports, regulatory submission sections, and peer-reviewed publications.
• Maintain instruments, troubleshoots instrumental/experimental problems, and order/maintain supplies.

Benefits

• comprehensive benefits, which vary by location. In the U.S., benefits may include health and wellness programs (including medical, dental, vision, life, and disability insurance), fitness centers, 401(k) company match, family support benefits, equity awards, annual bonuses, paid time off, and paid leaves (e.g., military and parental leave) for eligible employees at all levels!

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level