Associate Scientist - In Vitro Neurobiology

Amgen • Thousand Oaks, CA

1d

About The Position

Join Amgen’s Mission of Serving Patients. Amgen’s cardiometabolic team is an innovative target discovery and translational research unit with a focus on identifying novel targets for development of game-changing therapies for patients with obesity and associated cardiometabolic diseases. We employ advanced computational biology, state-of-the-art discovery screening technologies, genetics, and human-centric cell systems to dissect pathophysiology and identify novel drug targets. This laboratory-based position will focus on cell culture support for the development of large-scale qualitative and quantitative functional genomics screens utilizing human derived central nervous system (CNS) models. There is an overarching aim to characterize genes and genetic regulatory circuits involved in the control of cellular metabolism and advance discovery projects by performing in vitro experiments under partial supervision.

Requirements

• Master’s degree and 1 year of related scientific experience OR Bachelor’s degree and 3 years of related scientific experience

Nice To Haves

• At least 3 years of mammalian cell culturing experience
• Demonstrated experience using assay technologies (e.g., luminescence, fluorescence, TR-FRET, AlphaLISA or high-content systems).
• Experience performing molecular biology experiments (such as RNA, DNA extraction, qPCR, cloning, CRISPR/Cas9-based genome editing) or performing antibody-based experiments (flow cytometry, ELISA, immunofluorescence imaging and western blot).
• Hands-on experience in handling samples in 96-well format is preferred
• Familiarity with Metabolic Disease Pathophysiology
• Strong organizational, analytical, and problem-solving skills
• Proficiency in GraphPad Prism, Microsoft Word, Excel, and PowerPoint

Responsibilities

• Work in an in vitro setting using aseptic technique, passaging, expansion, cryopreservation, assessment of cell-line quality, and cell characterization
• Maintain mammalian cell cultures derived from iPSC and/or primary neuronal or glial models
• Utilize functional cell-based assays and support in the development of immunoassays (western blot, ELISA, flow cytometry, immunofluorescence staining), fluorescent microscopy, and other imaging methods
• Operate and maintain standard lab equipment
• Document experimental plans and procedures timely with attention to detail
• Exhibit self-motivation and proactive attitude in designing and conducting experiments using state-of-the-art technologies
• With some supervision, plan, conduct or monitor experiments, record and organize data, analyze results, troubleshoot, and generate robust and reliable data

Benefits

• A comprehensive employee benefits package, including a Retirement and Savings Plan with generous company contributions, group medical, dental and vision coverage, life and disability insurance, and flexible spending accounts
• A discretionary annual bonus program, or for field sales representatives, a sales-based incentive plan
• Stock-based long-term incentives
• Award-winning time-off plans
• Flexible work models where possible.

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level
• Education Level: Associate degree
• Number of Employees: 5,001-10,000 employees