Associate Scientist, Cell Line Development

Denali Therapeutics•South San Francisco, CA

1d•$106,000 - $138,000

About The Position

Denali Therapeutics is seeking a highly motivated, collaborative scientist to join its Bioprocess Development team, with a focus on cell line development. The role contributes to development of a mammalian biotherapeutic production platform and generation of commercially viable cell lines for novel and complex therapeutic molecules, including bispecific antibodies, Fc fusion proteins, and enzyme fusions. The scientist will work with a multidisciplinary team and support pipeline projects through experimental execution, analysis, documentation, and cross-functional collaboration.

Requirements

BA/BS or MS degree in Molecular Biology, Biochemistry, Cell Biology, or a related field.
Technical proficiency in mammalian cell culture and upstream process development, preferably in an industrial laboratory.
At least 2 years of experience working in a laboratory environment.
Proficiency with protein characterization techniques such as Octet, SDS-PAGE, Caliper, and HPLC.
Ability to organize, analyze, and interpret experimental data, specifically using JMP and Excel.
Ability to work effectively and collaboratively with other scientists in cross-functional project teams.
Excellent oral presentation and written communication skills.

Nice To Haves

Experience in CHO cell line development for biologics in a CMC setting.

Responsibilities

Support development of Denali’s mammalian biotherapeutic production platform to generate commercially viable cell lines.
Execute cell line development activities, including transfection, selection, single-cell sorting, clone imaging, clone selection, and clone banking for pipeline projects.
Design, execute, and interpret cell line development experiments in collaboration with other scientists, including using JMP statistical software for design-of-experiments work and figure generation.
Write technical and summary reports, including cell line development reports used as source documents for regulatory filings.
Communicate findings effectively with cross-functional teams and participate in scientific discussions.
Maintain accurate and detailed laboratory notebooks and relevant documentation.
Cross-functionally support cell culture process development through bioreactor production runs and DOE-based screening experiments as needed.
Evaluate, optimize, and implement characterization assays such as qPCR, Western blots, and flow cytometry.