Analytical Development - Molecular Biologist

Eurofins•Boston, MA

3d•$35 - $41•Onsite

About The Position

This position will be supporting an Analytical Development client team responsible for the characterization and testing of raw materials, RNA drug substances, and LNP drug products at different stages of development. The successful candidate will work closely with Quality Control, Quality Assurance, Process Development, and R&D groups to advance preclinical gene editing candidates and support a growing gene editing pipeline. We are seeking a skilled and motivated Analytical Development scientist to join a dynamic team focused on analytical assays for nucleic acid quantitation and next-generation sequencing (NGS). The position involves developing and executing analytical methods for non-GMP development studies, including comparability assessments, process development, stability testing, and product characterization. The candidate must be motivated, detail-oriented, and capable of supporting both routine testing activities and method development efforts.

Requirements

B.S./M.S. in Biological Sciences/Pharmaceutical Sciences, Biochemistry, Chemistry, Analytical Chemistry, or related field with at least 3 years of relevant laboratory industry experience.
Proficiency with molecular biology techniques including DNA/RNA electrophoresis methods, DNA amplification methods, various DNA/RNA enzymatic manipulations, cloning, DNA/RNA research grade purification techniques, DNA/RNA fragment size assessment techniques, and dPCR/ddPCR.
Some hands-on experience with NGS library preparation workflows for Illumina, Pacbio, and Oxford Nanopure sequencing platforms.
Familiarity with sequence analysis software such as SnapGene or equivalent.
Strong pipetting technique and meticulous attention to detail in laboratory settings.
Strong aseptic technique with hands-on mammalian cell culture experience.
Accurate and timely documentation practices with experience using an electronic laboratory notebook (ELN).
Authorization to work in the United States indefinitely without restriction or sponsorship.

Nice To Haves

Experience working with various biophysical instrumentation such as DLS, Osmometer, pH, and performing basic fluorescence-based assays.
Hands-on experience with Illumina sequencers such as Miseq/Nextseq, and Oxford Nanopore sequencer MinION.
Basic knowledge of Linux environment and conceptual understanding of NGS data analysis pipeline in the Linux command line environment.
Experience working with oligonucleotides or lipid nano particle (LNP) products and formulations.
Experience working in a QC GMP environment with strong documentation and pipetting skills.
Familiarity with gene editing techniques and related analytical methods.
Direct experience with statistical analysis tools.
Experience performing workflows using automation platforms.
Strong understanding of analytical methodology and development.

Responsibilities

Perform molecular biology techniques such as qPCR, d/ddPCR, and work with DNA/RNA material to perform extraction, purification, reverse transcription, amplification, fragment size assessments, electrophoresis methods, and enzymatic manipulation reactions.
Support and execute on activities to develop and qualify phase-appropriate analytical methods from the analytical research team intended for various activities including release and stability testing, product characterization, high-throughput analysis, and characterization of complex raw materials.
Potential execution of techniques including sterile tissue culture, maintenance of mammalian cell culture preps, and cell-based assays.
Potential execution of NGS (next generation sequencing) workflows including library preparation.
Collect, process, and analyze experimental data, ensuring accurate and timely documentation in an electronic laboratory notebook (ELN).
Review, interpret, and present data within the analytical team and cross-functionally.
Maintain laboratory equipment and order critical reagents.
Other duties as assigned.