Scientist I/II, In Vitro Pharmacology

Alloy Therapeutics • Waltham, MA

21d • $126,000 - $144,000 • Onsite

About The Position

We are seeking a highly motivated Scientist for in vitro screening and pre-clinical development to join our dynamic and fast-paced team. In this role, you will support the execution of in vitro functional cell-based assays to evaluate the potency, mechanism of action, and pharmacological properties of biologic therapeutics. This role requires exceptional organizational skills, a commitment to scientific excellence, and the ability to adapt to a dynamic environment. You will report to the Associate Director, In Vitro Pharmacology and will work collaboratively in a matrixed environment to drive biologics discovery while supporting rapid decision-making in a fast-moving biotech setting.

Requirements

• PhD in Immunology, Cell or Molecular Biology, or a related field, with 0-2 years (Scientist I) or 3-5 years (Scientist II) of relevant industry experience.
• Strong background in T cell biology research and functional evaluation in an academic or industry setting.
• Experienced in the development and execution of in vitro assays to characterize T cell activation and cytotoxicity (e.g. cell killing, cytokine release, proliferation, etc.) using both primary or genetically modified cell models.
• Flow cytometry and fundamental cell and molecular biology techniques required.
• Significant cell culture experience with primary and immortalized cell culture – isolation, handling, expansion and characterization of primary human immune cells.
• Experience handling human blood, leukopak processing, and PBMC isolations.
• Excellent organization, clear communication and teamwork abilities in a multidisciplinary setting.
• Thrives working on a team and collaborating with others to achieve project goals.
• Enjoys a dynamic and exciting work environment.
• Detail oriented, with rigorous scientific thinking and a passion for drug discovery to improve patients’ lives.

Nice To Haves

• Experience working with antibody-based therapeutics preferred.
• Direct experience with cell line engineering (e.g. CRISPR, lentiviral, etc) preferred.
• Experience with live cell imaging (e.g. Incucyte) a plus.

Responsibilities

• Develop and optimize cell-based activity assays for primary and/or engineered human immune cells (T cells, NK cells, macrophages, etc)
• Process human leukopaks, isolate and characterize human PBMCs.
• Expansion and analysis of primary or genetically modified primary T cells and other immune cells
• Utilize an array of analytical and molecular techniques to investigate the dynamics and responses of T cells and other immune cells to antibody-based therapeutics
• Execute cell-based functional assays using high-throughput approaches in primary cells, immortalized reporter cell lines, and other disease-relevant cellular systems.
• Contribute to study design, execution, and data analysis.
• Support day-to-day lab and experimental workflows.
• Effectively collaborate across divisional or departmental teams and with external partners to drive projects forward and obtain test materials and reagents.
• Present experimental data and findings to cross-functional teams and relevant stakeholders.
• Ensure timely and successful execution of project activities in alignment with project plans and priorities.
• Maintain detailed and accurate laboratory records in compliance with industry standards.
• Maintain laboratory organization and ensure compliance with safety protocols.

Benefits

• Competitive base and equity compensation commensurate with level of experience and independence
• 401(k) company match
• Generous personal and family medical, dental and vision benefits with 100% of premiums and deductibles covered
• Company-paid disability (STD, LTD) and life insurance
• Paid parental leave
• Family planning support up to $45,000 (e.g., IVF/PGT, adoption, surrogacy, egg retrieval)
• Unlimited PTO (paid time off) and flexible schedules
• Annual stipend for continuing education with commitment to your career through individualized professional development plan
• Wellness and Extensive Employee Assistance Program (EAP) including resources for mental wellness

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level
• Education Level: Ph.D. or professional degree