Research Lab Tech I, Hematology Hollenhorst Lab

Mass General Brigham•Boston, MA

1d•Hybrid

About The Position

The Hollenhorst Lab at Mass General Brigham applies innovative biochemical strategies to study clinically relevant questions regarding proteins that regulate bleeding and clotting. The goal is to further the understanding of basic biology of hemostasis and thrombosis with a focus on patient care. The lab is seeking an enthusiastic and motivated individual to perform experiments under the supervision of the principal investigator or a senior lab member. Key experimental tasks include western blots, immunoprecipitation, protein purification, and preparing samples for mass spectrometry analysis. The selected candidate will work with a high degree of independence, organize notebooks, manage data, and perform other relevant laboratory experiments related to platelets and coagulation factors. This position is particularly suitable for individuals seeking 1-2 years of research experience before applying to PhD and/or MD programs, offering direct mentorship and potential clinical shadowing opportunities. The Hollenhorst laboratory is located in the Harvard Institutes of Medicine building, within the Longwood Medical Area, and is affiliated with Brigham and Women’s Hospital Division of Hematology and Harvard Medical School, with collaborations across various institutions and access to state-of-the-art core facilities.

Requirements

Bachelor's degree in biology, chemistry, or a related scientific field.
A positive attitude and eagerness to learn.
Comfortable with working independently and in a team.
Good oral and written communication skills.
Ability to learn and perform a variety of laboratory protocols, to identify potential problems and troubleshoot solutions.
Strong organizational skills.
Ability to logically present data.
Sound judgment regarding time-management and laboratory safety.

Nice To Haves

Prior training in techniques such as western blot, immunoprecipitation, protein purification by liquid chromatography, flow cytometry, and preparation of samples for mass spectrometry analysis.
Previous experience (6 months+) with techniques in experimental biology, chemistry and/or biochemistry.

Responsibilities

Perform routine and non-routine experiments, which are moderate to complex, with increasing independence. These include western blot, immunoprecipitation, protein purification by liquid chromatography, flow cytometry, and preparation of samples for mass spectrometry analysis.
Process platelets and plasma samples from donors and patients.
Maintain long-term tissue cultures of human cell lines, monitor cells for viability and contamination.
Maintain a detailed electronic laboratory notebook to reproduce experiments precisely.
Consult with the principal investigator and implement adjustments in experimental design.
Prepare laboratory reagents and chemicals, organize storage.
Meticulously maintain and update databases of laboratory supplies and storage.
Order new supplies on time to maintain inventory.
Perform routine maintenance and calibration of laboratory equipment, if necessary.
Become proficient in Excel, FlowJo, and GraphPad Prism as needed analyze and critically evaluate data.
Present data and relevant research publications in lab meetings.
Help to prepare presentations and written articles for publication.
All other duties, as assigned.