Research Lab Tech I

Mass General Brigham•Boston, MA

1d•Onsite

About The Position

The Kleinstiver Laboratory in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) is seeking motivated and enthusiastic candidates to join their team. The research group engineers and optimizes genome editing technologies, including CRISPR-Cas enzymes, base editors, prime editors, click editors, CRISPR-associated transposases (CASTs), transposons, recombinases, and other novel non-CRISPR editing platforms. The goal is to apply these technologies for biomedical research and to treat human diseases. The lab focuses on optimizing properties such as efficiency, safety, versatility, programmability, and precision of these tools through methods like rational design, directed evolution, structure-guided engineering, and machine learning. The optimized tools are then translated into pre-clinical models and primary cell types to explore their potential to correct pathogenic mutations for a diverse set of diseases, including liver-based disorders, inborn errors of immunity, neurological and neuromuscular conditions, and deafness. The candidate for the Research Technician I position will play an integral role in projects related to genome editing technology development, protein engineering method development, and expediting the clinical application of these tools. Candidates ideally have interests in genome engineering, technology development, the creation of genetic medicines, and other related fields.

Requirements

Bachelor's Degree in a Related Field of Study (experience can be accepted in lieu of a degree)
Prior laboratory experience, either through academic coursework, internships, or employment
Strong theoretical and practical knowledge of laboratory techniques and procedures
Familiarity with common laboratory equipment and instruments used in research experiments
Proficiency in data collection, analysis, and scientific software tools
Excellent attention to detail
Organizational skills
Ability to multitask and prioritize tasks effectively
Strong problem-solving skills
Ability to troubleshoot technical issues
Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges

Nice To Haves

1-2 years preferred prior laboratory experience
Interests in genome engineering, technology development, the creation of genetic medicines, and other related fields
Knowledge or knowhow of genome editing technologies

Responsibilities

Become proficient in routine molecular biology (PCR, recombinant DNA cloning, bacterial transformations, etc.)
Become proficient in biochemical techniques
Become proficient in mammalian cell culture
Become proficient in assessment of genome editing technologies in bacteria and mammalian cells
Become proficient in DNA sequencing (Sanger and NGS)
Design and execute experiments
Analyze and visualize data
Assist with routine laboratory tasks (organizing, ordering, stocking, etc.)
Prepare reagents and solutions
Develop and optimize genome editing assays
Record experimental details and results in an electronic notebook
Manage time effectively
Communicate effectively
Present summaries of work or scientific papers in lab meeting and journal club, respectively
Perform laboratory experiments following established protocols and procedures
Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines
Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed
Record experimental data accurately and maintain organized records of procedures, protocols, and results
Compile and analyze data using appropriate statistical and analytical methods
Assist in data interpretation and presentation, contributing to research findings and reports