Associate Scientist-Proteomics

University of Miami Miller School of Medicine•Miami, FL

65d•Onsite

About The Position

The Associate Scientist, SOM supports the University's research goals and objectives by performing laboratory experiments in his/her areas of interest, expertise, or assignment. Moreover, the incumbent performs various analytical procedures involving method development and application development and synthesis. The Sylvester Comprehensive Cancer Center is looking for an Associate Scientist to join the newly established Proteomic Shared Resources, to apply state of the art LC-MS based methods and techniques for quantitative proteomics. As an Associate Scientist, you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. In addition, you will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology.

Requirements

Doctorate degree - Philosophy (PhD) required
Minimum 5 years of relevant experience required
Learning Agility: Ability to learn new procedures, technologies, and protocols, and adapt to changing priorities and work demands.
Teamwork: Ability to work collaboratively with others and contribute to a team environment.
Technical Proficiency: Skilled in using office software, technology, and relevant computer applications.
Communication: Strong and clear written and verbal communication skills for interacting with colleagues and stakeholders.
Ph.D. in Science or Engineering (Biochemistry, Bioengineering, Biology, Chemistry, or related field)
5 years of related experience with mass spectrometry instruments which includes 2 years of experience in proteomics mass spectrometry applications.
Hands-on experience operating and/or maintaining LC-MS instruments and supporting equipment (including auxiliary systems such as chillers, controls, electronics, pumps, vacuum systems, etc.)
Strong interest in handling, processing, and analyzing high-throughput datasets.
Motivation to constantly develop skills and expertise.
Ability to work both independently and as a team member while engaging in the organization of the proteomics core.
Excellent analytical, communication, problem solving, and organizational skills.
excellent written and oral communication skills.
Established track record of publications and demonstrated ability to complete projects.
Capability to learn complex tasks with instruction and to use experience to perform a variety of new techniques.

Nice To Haves

expertise in one or more of the following technical areas: quantitative proteomics, single cell proteomic analysis, global proteome profiling.
Previous hands-on operation of Thermo Tribrid Orbitrap LC-MS Systems (e.g., Fusion, Eclipse, Ascend).

Responsibilities

Contributes to basic and applied research activities including authorship of scientific publications, technical and agency reports, and patent preparations.
Publishes in refereed journals in collaboration with department leadership or independently.
Supervises research employees and non-exempt staff.
Writes extramural proposals with approval of senior managing staff.
Creates and conducts experiments and analyzes results.
Works with other researchers to use and develop end products.
Provides ready access to all experimental data for the faculty researchers, supervisors, and other relevant staff.
Requests or acquires equipment and supplies for assigned project(s).
Applies for grants to continue research and prepares materials for submission to granting agencies and foundations.
Adheres to University and unit-level policies and procedures and safeguards University assets.
Primary responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications.
Will manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks.
Prepare protein samples from a wide range of specimen (e.g., cells, tissues, biofluids, and SDS Page gels)
Implement of both bottom-up & top-down proteomic approaches.
Responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation).
Perform relative quantification of proteins by label-free and stable-isotope labelling strategies (e.g., SILAC, TMT).
Responsible for structural proteomics (e.g., cross-linking, pull-down experiments).
Will conduct Single cell proteomics analysis.
Intact protein analysis & MW determination.
Use of proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis.
Responsible for operation, maintenance, and troubleshooting of Orbitrap Ascend Tribrid Mass Spectrometry and other laboratory equipment as needed.
Assesses experimental results and troubleshoot methods and instruments to optimize research protocols.
Collaborates efficiently with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues.