Technical Associate I, Weiss lab
About The Position
TECHNICAL ASSOCIATE 1 , Biological Engineering–Synthetic Biology Center (SBC), Weiss lab, will be aiding research staff in ongoing research projects. Research areas supported include iPSC differentiation to specific cell fates and heterogeneous tissues (including organoids/liver organoids), genetic programming of organoids to advance tissue maturation and drug development, and engineering gene-delivery systems and genomic integration strategies in human cells. Lab work includes mammalian and/or organoid cell culture, molecular cloning (genetic engineering), transfection, lentivirus production, lentivirus transduction, immunofluorescent staining, flow cytometry, microscopic image acquisition, ELISA, Droplet Digital PCR, and data preparation. Will be working with basic aseptic molecular and cellular biology and mammalian cell culture techniques and prepare biweekly progress reports with detailed analysis of experimental results, issues, and updates. Some work requires the use of lentiviruses in a BL2 or BL2+ setting. Will sterile passage and maintenance of cell lines; prepare media, bacterial, and mammalian cell stocks in a sterile environment; organize laboratory supplies and reagents; follow complex instructions and demonstrate a history of carefully documenting work; and plot and analyze data and present to the project leader and faculty supervisor for review.
Requirements
- Bachelor's degree in Biology, Bioengineering, Biochemistry or a closely related field
- A minimum of two year’s of laboratory technician experience
- Experience in basic aseptic molecular and cellular biology and mammalian cell culture technique
- Experience in basic chemistry/biology lab techniques
- Laboratory experience with basic experimental procedures
- Experience with DNA cloning, handling RNA, and transfection techniques
- Knowledge of imaging technique, including flow cytometry, confocal and immunofluorescence imaging
- Excellent written and verbal communication skills
- Solid organizational and planning skills
- Self-motivated and detail-oriented
- Excellent interpersonal skills
- Ability to work both independently and as part of a team
- Ability to follow complex instructions, plan and troubleshoot experiments independently, multitask, carefully document work in written records, and work in a fast-paced, dynamic research environment
- Ability to work with automated and high-throughput systems
- Ability to maintain confidentiality and security of project information
Nice To Haves
- Experience with DNA and RNA handling/transfection, lentivirus production and transduction, stem cell culture, and organoid culture is desirable
Responsibilities
- Aiding research staff in ongoing research projects
- Mammalian and/or organoid cell culture
- Molecular cloning (genetic engineering)
- Transfection
- Lentivirus production
- Lentivirus transduction
- Immunofluorescent staining
- Flow cytometry
- Microscopic image acquisition
- ELISA
- Droplet Digital PCR
- Data preparation
- Prepare biweekly progress reports with detailed analysis of experimental results, issues, and updates
- Sterile passage and maintenance of cell lines
- Prepare media, bacterial, and mammalian cell stocks in a sterile environment
- Organize laboratory supplies and reagents
- Follow complex instructions and demonstrate a history of carefully documenting work
- Plot and analyze data and present to the project leader and faculty supervisor for review
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Number of Employees
5,001-10,000 employees
