Staff Scientist

University of Rochester

4d•$29 - $41•Onsite

About The Position

As a community, the University of Rochester is defined by a deep commitment to Meliora - Ever Better. Embedded in that ideal are the values we share: equity, leadership, integrity, openness, respect, and accountability. Together, we will set the highest standards for how we treat each other to ensure our community is welcoming to all and is a place where all can thrive. The candidate will be involved in a project investigating Ca2+ transfer at membrane contact sites. Will have experience in fluorescence imaging including widefield, TIRF, confocal or Multiphoton imaging to analyze the function of proteins at contact sites. Will perform contemporary biochemical and molecular biological techniques including transfection of cDNA constructs, immunolocalization and purification of proteins. Will assist with training of lab personnel including graduate students and postdoctoral fellows. Will assist with managing projects, including writing paper drafts and assist in the preparation of data for grant submission. Ability to work in a lab team environment with a diverse group of personnel is essential. This is a TAR position. The University of Rochester is committed to fostering, cultivating, and preserving an inclusive and welcoming culture to advance the University’s Mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion, creed, sex, sexual orientation, citizenship status, or any other characteristic protected by federal, state, or local law (Protected Characteristics). This commitment extends to non-discrimination in the administration of our policies, admissions, employment, access, and recruitment of candidates, for all persons consistent with our values and based on applicable law. Learn. Discover. Heal. Create. Located in western New York, Rochester is our namesake and our home. One of the world’s leading research universities, Rochester has a long tradition of breaking boundaries—always pushing and questioning, learning and unlearning. We transform ideas into enterprises that create value and make the world ever better. If you’re looking for a career in higher education or health care, the University of Rochester may offer the perfect opportunity for your background and goals. At the University of Rochester, we are committed to fostering, cultivating, and preserving an inclusive and welcoming culture and are united by a strong commitment to be ever better—Meliora. It is an ideal that informs our shared mission to ensure all members of our community feel safe, respected, included, and valued.

Requirements

PhD in Physiology, Biochemistry, Biotechnology, or a related field required
2 years of postdoctoral training, or equivalent combination of education and experience required
Required technical skills included advanced molecular biology techniques, protein biochemistry, fluorescence microscopy imaging and some experience with electrophysiology
Ability to work in a lab team environment with a diverse group of personnel is essential.

Responsibilities

The candidate will be involved in a project investigating Ca2+ transfer at membrane contact sites.
Will have experience in fluorescence imaging including widefield, TIRF, confocal or Multiphoton imaging to analyze the function of proteins at contact sites.
Will perform contemporary biochemical and molecular biological techniques including transfection of cDNA constructs, immunolocalization and purification of proteins.
Will assist with training of lab personnel including graduate students and postdoctoral fellows.
Will assist with managing projects, including writing paper drafts and assist in the preparation of data for grant submission.
Molecular Biology: Must be proficient in RNA/DNA extraction, RT-qPCR, site directed mutagenesis, transfection and transduction. Propagating plasmids. Must be proficient in CRISPR technology to generate Knock out/in genomic variants.
Protein Biochemistry: Must be proficient in protein isolation, protein labelling, SDS PAGE, Native PAGE, immunopurification, Immunoblotting, Immunofluorescence and maintenance of cells in culture.
Ca2+ imaging: Work in an independent manner with minimal supervision. Utilize cultured cells expressing IP3R constructs for Ca2+ imaging experiments. BE willing to optimize protocols for different constructs dependent on functional phenotype. Must be adaptable to experimental needs. Willing to appropriately analyze and interpret data in preparation for presentations and publications. Must be willing to keep current with advances in. concepts and technologies.
Training responsibility: Helps to interview and train laboratory personnel and rotating graduate student on laboratory and departmental-shared equipment and laboratory procedures. May supervise and evaluate the work of other laboratory personnel. Counsels laboratory personnel on technical issues, problem solving, or as issues arise. Develop and install operating procedures for the laboratory, operate complex equipment. Perform routine equipment maintenance and troubleshooting
Research analysis, Presentation and Searches. Fluent in GraphPad Prizm/Excel or similar. Prepare literature searches as needed. Analyze and present findings to PI and at laboratory meetings
Other job duties as assigned.