Staff Research Associate III - Ophthalmology
About The Position
Under the direction of the Principal Investigator (PI), the Staff Research Associate III (SRA III) is responsible for executing cellular and molecular techniques as part of the basic, translational and clinical research. Duties include the cell culture of primary cells and cell lines as well as other molecular biology techniques including but not limited to cloning, mutagenesis, sequencing, polymerase chain reaction (PCR), Western Blot, immunohistochemistry/immunocytochemistry, and cryosectioning. The SRA III will perform imaging acquisition and imaging and data analysis, keep good documentation records in compliance, assist with pre-clinical experiments and actively participate in qualifications and clinical manufacturing part of a Phase I clinical trial. The SRA III will also be in charge of lab management and lab maintenance including but not limited to overseeing lab activities ensuring that a safe, clean, organized, well equipped/stocked and compliant environment is maintained.
Requirements
- Bachelor's degree in life science (not including psychology) and or equivalent experience and/or training
- Knowledge of molecular biology techniques
- Skills and extensive experience in human and mouse cell culture technique
- Knowledge of cell culture, including thawing cells, culturing cells and freezing cells
- Skills in cryosection
- Skills in isolating and purifying high quality DNA and RNA
- Skill in subcloning of cell lines
- Ability to use molecular biology software
- Ability and experience to perform immunohistochemistry
- Ability to perform protein analysis by gel electrophoresis and Western Blots
- Experience with tissue and cell imaging and analysis
- Ability to establish and maintain records and supply stocks
- Skill in maintaining the lab and equipment in working condition
- Ability to establish and maintain cooperative working relationships with co-workers
- Demonstrated experience using general lab equipment such as pipettes, centrifuges, balances, incubators, thermal cyclers, dry blocks, shakers, autoclaves, water baths, liquid nitrogen tanks, DNA synthesizer, pH meter, etc.
- Ability to work with mice, setting up breeding pairs and recording births and weaning animals
- Ability to plan, execute and coordinate all the GMP/GLP activities related to the pre-clinical and Phase I clinical projects in collaboration with the research teams
- Knowledge of GLP and GMP
- Ability to manage and maintain the lab ensuring that a safe, clean and compliant environment is maintained
- Ability to work on multiple projects, often changing from one task to another
- Ability to establish and maintain cooperative working relationships with personnel both in and outside of the laboratory group
- Communicate clearly, concisely and accurately in verbal and written formats
- Ability to summarize and present data
Responsibilities
- Executing cellular and molecular techniques
- Cell culture of primary cells and cell lines
- Cloning
- Mutagenesis
- Sequencing
- Polymerase chain reaction (PCR)
- Western Blot
- Immunohistochemistry/immunocytochemistry
- Cryosectioning
- Imaging acquisition and imaging and data analysis
- Keep good documentation records in compliance
- Assist with pre-clinical experiments
- Actively participate in qualifications and clinical manufacturing part of a Phase I clinical trial
- Lab management and lab maintenance
- Overseeing lab activities ensuring that a safe, clean, organized, well equipped/stocked and compliant environment is maintained
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
Industry
Hospitals
Number of Employees
5,001-10,000 employees
