Scientist, Single Cell Technology Development & Translational Genomics

CZ Biohub•New York, NY

53d•$120,000 - $189,000•Onsite

About The Position

The Laboratory of Immunogenomics at Biohub studies the non-coding regulatory genome to understand and address immune dysfunction in diseases like cancer, autoimmune disorders, and aging. We focus on enhancers-non-coding, highly cell-type-specific transcriptional regulatory elements-and their role in shaping immune responses. We develop and utilize genomic technologies, including bulk and single-cell nascent RNA sequencing, genome editing, immune engineering, and CRISPR-based functional screens in patient biopsies, organoid systems, and mouse models. Through computational analysis integrating machine learning and AI, we map enhancer-gene networks and identify disease-driving elements. Our goal is to advance enhancer-guided precision genomic medicine for diseases involving immune dysfunction. We are seeking a highly motivated Scientist to lead the development of a high-throughput adaptation of a newly established single-cell nascent RNA sequencing method (scGRO-seq) and apply it to patient biopsies and organoid models. The role involves designing, optimizing, and benchmarking cutting-edge single-cell genomics workflows, integrating them with perturbation-based assays, and generating datasets that drive transformative insights into immune regulation. For candidates with demonstrated technical expertise and leadership ability, this position offers the opportunity to build and lead a small team (one to two research associates) to implement the assay, conduct perturbation experiments, and oversee data generation. Once the workflow is established, this position will direct experimental execution, coordinate large-scale data acquisition, and collaborate on downstream analyses with computational biologists to deliver actionable translational insights. This role is ideal for a post-PhD scientist with a proven track record in single-cell biology who excels at the intersection of technological innovation, complex biology, and computational analysis. You will join a highly collaborative team working toward a shared vision. The position offers rich opportunities for professional growth-developing leadership, mentorship, and multidisciplinary expertise-equipping the candidate to thrive at the cutting-edge of single-cell immunogenomics for future academic or industry roles.

Requirements

2+ years of postdoctoral or equivalent post-PhD experience in academic or industry settings.
PhD in Molecular Biology, Systems Biology, Genetics, Immunology, Cancer Biology, Biomedical Engineering, or related field.
Proven expertise in single-cell or spatial omics technology development, ideally in RNA biology or immune engineering.
Demonstrated success in genomic assay development from concept to implementation.
Strong background in immune cell biology, innate and adaptive immunity, and hematopoietic stem/progenitor cell culture and reprogramming.
Experience designing and executing CRISPR screens and perturbation assays (genetic or chemical/drug).
Strong problem-solving skills, independence, and the ability to troubleshoot complex workflows.
Excellent communication skills and proven ability to work in collaborative, multidisciplinary teams.

Nice To Haves

Computational fluency in genomic data analysis; ability to work with bioinformatics pipelines for single-cell and bulk datasets.
Experience with functional genomics in immune cells.
Familiarity with 3D culture systems or hydrogels for immune cell studies.
Experience integrating multi-modal single-cell datasets (RNA, ATAC, proteomics).
Working knowledge of Linux/HPC environments and single-cell analysis tools (e.g., Seurat, Scanpy).

Responsibilities

Assay development: Design, optimize, and scale high-throughput single-cell/nuclei extraction, barcoding, and library preparation workflows for scGRO-seq.
Perturbation integration: Combine scGRO-seq with CRISPR-based enhancer perturbation, drug treatments, and multi-omics profiling.
Molecular biology techniques: Execute advanced methods including assay design, cell manipulation, RNA/DNA isolation, PCR/qPCR, NGS library construction, high-dimensional flow cytometry, and sequencing.
Translational applications: Adapt and validate protocols in patient biopsies and 3D organoid models, with emphasis on reproducibility and sensitivity.
Immune cell engineering: Isolate, culture, and reprogram hematopoietic stem/progenitor cells (HSPCs) and primary immune subsets.
Data integration: Partner with computational biologists to process and interpret large-scale single-cell datasets (RNA, chromatin, proteomics) using established and custom pipelines (e.g., Bowtie, STAR, Seurat, Scanpy).
Collaboration & dissemination: Collaborate across disciplines to investigate regulatory genomics in immune dysfunction, tumor-infiltrating immune cells, and autoimmune disease variants. Contribute to publications, preprints, conference presentations, and grant proposals.

Benefits

Provides a generous employer match on employee 401(k) contributions to support planning for the future.
Paid time off to volunteer at an organization of your choice.
Funding for select family-forming benefits.
Relocation support for employees who need assistance moving

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