Scientist I, Cell-Based Assays

Prime Medicine • Cambridge, MA

14d • Onsite

About The Position

Prime Medicine is a leading biotechnology company dedicated to creating and delivering the next generation of gene editing therapies to patients. The Company is leveraging its proprietary Prime Editing platform, a versatile, precise and efficient gene editing technology, to develop a new class of differentiated, one-time, potentially curative genetic therapies. Designed to make only the right edit at the right position within a gene while minimizing unwanted DNA modifications, Prime Editors have the potential to repair almost all types of genetic mutations and work in many different tissues, organs and cell types. Prime Medicine is currently progressing a diversified portfolio of investigational therapeutic programs organized around our core areas of focus: hematology, immunology & oncology, liver and lung. Across each core area, Prime Medicine is focused initially on a set of high value programs, each targeting a disease with well-understood biology and a clearly defined clinical development and regulatory path, and each expected to provide the foundation for expansion into additional opportunities. For more information, please visit www.primemedicine.com. Position Overview We are seeking a Scientist to join our cystic fibrosis team to lead the assessments of in vitro and in vivo delivery to lung epithelial cells. This role will utilize primary 2D and 3D mammalian cell cultures and mouse tissues to evaluate AAV- and LNP-mediated delivery to target cells. This work will guide the selection of lead candidate components for both AAV and LNP drug candidates. We are looking for curious problem solvers who enjoy working on fast-paced, exciting projects, working within a team, and are passionate about developing gene editing technologies to improve human health. The ideal candidate will be on site 5 days a week in Cambridge, MA.

Requirements

• PhD in cell biology, immunology, genetics, developmental biology, or related field with 0-2+ years of relevant experience (or BS+8, MS+6).
• Experience with complex non-immune primary mammalian cell cultures, including cell differentiation and use of transwells.
• Experience dissociating tissues for downstream cell-based assays.
• Familiarity/experience with molecular biology techniques (e.g., qPCR, DNA sequencing, western blot)
• Strong organizational and critical thinking skills, attention to detail, and ability to work both independently and as part of a collaborative team.
• Excellent communication and interpersonal skills to thrive in a cross-functional setting.

Nice To Haves

• Subject matter expertise in lung biology, drug development for lung diseases, and considerations for in vivo delivery of test articles to the lung.
• Hands-on experience with in vivo lung delivery (intratracheal and nebulization).
• Hands-on experience with flow cytometry panel design, sample preparation, instrument operation, and data analysis.
• Experience with cell sorting.
• Development of cell-based immunogenicity assessments such as intracellular cytokine staining and ELISPOT.

Responsibilities

• Utilize 2D and 3D epithelial cell cultures to assess delivery and potency of AAV- and LNP-based discovery candidates.
• Differentiating and maintaining multiple primary epithelial cell cultures.
• Preparing/dissociating cells and tissues into single cell suspensions to perform cell-based analyses using flow cytometry and molecular biology techniques.
• Collaborate cross-functionally with internal teams on study design, execution, data collection, data analysis, and interpretation.
• Present data in written reports and oral presentations.

Benefits

• Prime Medicine offers a comprehensive Total Rewards package with robust health, financial, and wellness benefits—including medical, dental, vision, life insurance, a 401(k) match, and equity programs—along with generous paid time off, wellness days, and company-wide recharge breaks.
• Our commitment to employee well-being reflects our belief that when we care for our people, we strengthen our ability to put patients first and make meaningful impact together.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree
• Number of Employees: 101-250 employees