Scientist, Bioanalytical Assays

Flagship Pioneering, Inc.•Cambridge, MA

1d•$100,000 - $143,000

About The Position

We are seeking a highly motivated and experienced Scientist with expertise in bioanalytical assays. This full-time bench scientist position requires expertise in bioanalytical platforms, LNPs, nucleic acid therapies, and biodistribution studies. This individual will have the opportunity to directly contribute to the bioanalytical assay development for platform and therapeutic areas, and work in a dynamic and fast-paced entrepreneurial environment as part of a highly collaborative team.

Requirements

Ph.D. in biochemistry, pharmaceutical sciences, cell biology or related field with 2 plus years of industry experience
Proficiency in ddPCR, ligand binding assays and histological techniques
Experience developing bioanalytical methods to measure drugs and biomarkers for LNP delivery of nucleic acid cargoes
Strong communication, presentation and interpersonal skills
Attention to detail, strategic thinking, problem solving, and decision-making skills

Nice To Haves

Experience with non-viral delivery technologies and nucleic acid modalities is preferred

Responsibilities

Design, develop, optimize, and execute bioanalytical assays to support nonclinical studies, including PK, PD, biomarker, and immunogenicity endpoints
Execute ligand-binding assays and cell-based assays, ensuring robust assay performance, data quality, and compliance with applicable guidelines
Design, optimize, and execute biodistribution studies (flow cytometry and histological techniques) for LNP/nucleic acid therapies
Collaborate closely with CMC, molecular biology and cell biology teams to screen cargoes (immunogenicity assessment etc.) and ensure timely delivery of high-quality data and summarize results
Establish and execute various cell-based assays to assess immunogenicity to nucleic acid cargoes and establish release criteria for preclinical payloads
Design, establish and execute assays for assessing various in vivo immunogenicity predictors for LNP/nucleic acid therapies including but not limited to biomarkers, cytokines, complement activation and anti-drug antibodies (ADA)
Execute test procedures such as sample extraction and measurement by flow cytometry, ELISA, MSD, Luminex, bDNA, ddPCR, RNAscope and perform data interpretation
Analyze and summarize scientific data and maintain timely documentation and completion of electronic lab notebooks in compliance with industry guidelines
Present at project team meetings and to relevant stakeholders