Glyphic Biotechnologies-posted 2 days ago
Full-time • Mid Level
Onsite • Berkeley, CA
11-50 employees

At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$50M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing. We are seeking an experienced, hands-on Assay Development Scientist to design and perform experiments that enable the next generation of sensitive, accurate, and scalable single-molecule protein sequencing. Reporting to the Director of Assay Development, you will leverage your molecular biology expertise in a cross-functional environment at the interface of molecular biology, chemistry, bioinformatics, and automation. The ideal candidate thrives at the bench in a dynamic research team and contributes to intellectually stimulating and highly collaborative projects. This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.

  • Plan, and execute experiments to improve the efficiency, accuracy, and scalability of Glyphic’s protein sequencing by expansion (ProSE) process
  • Propose, design, and test novel analytical approaches to optimize and build predictive understanding of critical components of the ProSE sequencing process including sample preparation, molecular expansion chemistry, and automation integration
  • Proactively identify, communicate, and help address experimental risks or resource gaps, such as reagent supply or instrument issues
  • Collaborate with scientists on the Assay Development team to ensure successful delivery of team goals and prioritize experiments accordingly
  • Collaborate with scientists in Chemistry, Bioinformatics, Process Development, and Engineering teams to assist and contribute to cross-functional projects
  • Collect, manage, and analyze data from molecular biology and protein sequencing experiments
  • Keep a detailed electronic lab notebook on Confluence
  • Communicate and present results in a timely manner
  • BS/MS/PhD with 6+/4+/2+ (for Scientist II) or BS/MS/PhD with 4+/2+/0+ (for Scientist I) years of post-graduate experience in molecular biology, biochemistry, chemistry or related fields
  • Experience running and troubleshooting protocols for highly sensitive assays (PCR-free sequencing, single cell assays, NULISA)
  • Expertise and sound conceptual understanding of DNA-based assays, including ligation, hybridization capture, qPCR, and gel electrophoresis
  • Experience with and conceptual understanding of bead-based DNA or protein purification and QC methods such as gel extraction, Tapestation, Bioanalyzer, SeqStudio, and UV-vis absorbance measurements
  • Familiarity with chemical safety protocols for handling reagents such as strong acids and bases or working in anoxic and anhydrous environments (e.g., glove boxes)
  • Academic exposure to organic chemistry
  • Detail-oriented, organized, and able to keep accurate lab records
  • Strong written and verbal communication skills
  • Ability to work independently and as a team member
  • Motivated, enthusiastic, and proactive individual
  • Experience with chemical functionalization of biomolecules or surfaces (e.g. EDC, NHS, Click)
  • Experience with data analysis using GraphPad Prism, Excel, and/or similar programs
  • Experience with bead-based automation on instruments such as KingFisher or Hamilton
  • Familiarity with HPLC for biomolecule purification and validation is a plus
  • Familiarity with emerging trends and techniques in proteomic measurements is a plus
  • Employee Stock Option Plan
  • 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
  • Employer Retirement Contributions to 401(k)
  • Generous Paid Time Off
  • Paid Maternity and Paternity Leave
  • Health & Wellbeing Program
  • Office Snacks and Beverages
  • Regular Team Bonding Activities
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