Researcher I

University of Wisconsin Madison • Madison, WI

1d • $50,000

About The Position

The Engin Lab is looking for a Researcher to contribute to the lab's research efforts focused on understanding the mechanism of type 1 diabetes. The work in the lab involves molecular biology, cell culture, microscopy, and mouse metabolic and pharmacological treatment experiments. The successful candidate will conduct independent research under the guidance of the PI and assist with the development of lab experiments involving molecular biology (PCR, quantitative PCR, flow cytometry, mammalian cell culture, cell biology (immunostaining, microscopy). The candidate will be expected to maintain genetically manipulated mouse lines and to characterize metabolic phenotypes in these strains. Procedures include mouse husbandry, PCR-based genotyping, histology, immunohistochemistry, and metabolic phenotyping (measurement of body weight and blood glucose, collection of tissue and blood samples, treatment of mice with various drugs). The Researcher will prepare figures of their experiments/findings and present their data in weekly lab meetings and potentially in other local or national seminars/conferences. Additional responsibilities include ordering supplies when necessary, maintaining laboratory records, preparation of some laboratory reagents, maintaining laboratory equipment, supervising student workers, and training laboratory staff in techniques and protocols. It is expected that the Researcher will be an active participant in the lab, attend and present at regular group meetings, and prepare portions of scientific manuscripts on which she/he will be a co-author. More information about our research and publications can be found at enginlab.org .

Requirements

• At least 3 years of full-time laboratory experience in an academic environment post bachelor's.
• Experience with mammalian cell culture
• Excellent record keeping and organizational skills

Nice To Haves

• Experience working with mouse colonies, genotyping by PCR, administration of drugs
• Experience with siRNA, shRNA, gene knockout or over expression in mammalian culture
• Familiarity with molecular biology techniques such as PCR, western blot, DNA and RNA isolation
• Imaging (confocal)
• Immunofluorescence, immunostaining
• Flow cytometry

Responsibilities

• Conducts literature reviews, prepares reports and materials, and disseminates information to appropriate entities and assists with publications
• Identifies concerns and recommends solutions to leadership
• Assists in developing grant applications and proposals to secure research funding
• Assists with the development of research methodologies and procedures
• Collects and analyzes basic research data, conducts experiments and interviews, and documents results according to established policies and procedures
• Trains individuals and groups on equipment operations, research protocols, and techniques within a specialized laboratory or research area

Benefits

• Employees in this position can expect to receive benefits such as generous vacation, holidays, and sick leave; competitive insurances and savings accounts; retirement benefits.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level