RESEARCH TECHNICIAN II

Duke Careers • Durham, NC

3d • $19 - $29

About The Position

The Lee lab in the Department of Radiation Oncology is recruiting a Research Technician to conduct molecular biology, cell biology, histology, and animal experiments. This person will work on NIH-funded projects that aim to dissect mechanisms underlying the development of normal tissue injury induced by ionizing radiation in the gastrointestinal (GI) tract and oral mucosa. Complementary approaches will be used to study radiation-induced injury and regeneration of epithelial cells in organoids and genetically engineered mouse models.

Requirements

• Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
• None required above education/training requirement.
• OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Responsibilities

• Perform various molecular biology and histology experiments, including but not limited to: DNA/RNA/Protein extraction, qRT-PCR, immunoblotting, immunohistochemistry, immunofluorescent staining, and flow cytometry
• Perform animal experiments studying radiation responses in vivo
• Perform various cell biology experiments to study the response of intestinal organoids and oral mucosal organoids to radiation in vitro
• Assist with maintaining lab mouse colony: set up crosses, perform tail clip biopsies, mark mice (tattoo, ear tag, etc.), and wean pups
• Maintain an organized and complete lab notebook
• Set up instruments, materials, and apparatus, and operate standard laboratory equipment required for specific tests
• Collect and report raw data to the PI
• Teach new lab members scientific techniques that you are familiar with
• Perform other related duties incidental to the work described herein
• Help provide a collegial environment for the laboratory
• Meet with the PI weekly to plan experiments, troubleshoot problems, and discuss results
• Must be flexible to work on weekends or shifts
• Must be detail-oriented, well-organized, and highly collaborative with strong communication skills and the ability to work in a fast-paced, interactive team environment

Benefits

• Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family- friendly and cultural programs to eligible team members.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Number of Employees: 5,001-10,000 employees