Research Lab Tech I

Mass General Brigham•Boston, MA

1d•Onsite

About The Position

The Kleinstiver Laboratory (www.kleinstiverlab.org) in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) is seeking motivated and enthusiastic candidates to join our team. Our research group engineers and optimizes genome editing technologies to apply them for biomedical research and to treat human diseases. We pursue protein and nucleic protein engineering methods to impart desirable properties into CRISPR-Cas enzymes (e.g. Cas9, Cas12, etc.), for next-generation technologies capable of installing single letter DNA edits (e.g. base editors), for polymerization-based DNA writing approaches (e.g. prime editors, click editors, etc.), for various editors capable of larger sequence edits (e.g. CRISPR-associated transposases (CASTs), transposons, recombinases, etc.) and other novel non-CRISPR editing platforms. Examples of key properties that seek to optimize include their efficiency (on-target activity), safety (reducing off-target activity), versatility and programmability (targeting range / ability to access the genome), precision (their edit outcomes), and other characteristics. Optimized proteins are developed via rational design, directed evolution, structure-guided engineering, machine learning, and additional approaches. We then translate these optimized tools into pre-clinical models and primary cell types to explore their potential to correct pathogenic mutations for a diverse set of diseases including liver-based disorders, inborn errors of immunity, neurological and neuromuscular, deafness, etc. The candidate will play an integral role in projects related to genome editing technology development, protein engineering method development, and expediting the clinical application of these tools.

Requirements

Bachelor's Degree in a Related Field of Study or equivalent experience
Prior laboratory experience, either through academic coursework, internships, or employment
Strong theoretical and practical knowledge of laboratory techniques and procedures
Familiarity with common laboratory equipment and instruments used in research experiments
Proficiency in data collection, analysis, and scientific software tools
Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively
Strong problem-solving skills and the ability to troubleshoot technical issues
Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges

Nice To Haves

Interests in genome engineering, technology development, the creation of genetic medicines, and other related fields
1-2 years of prior laboratory experience
Knowledge or knowhow of genome editing technologies

Responsibilities

Become proficient in routine molecular biology (PCR, recombinant DNA cloning, bacterial transformations, etc.)
Become proficient in biochemical techniques
Become proficient in mammalian cell culture
Become proficient in assessment of genome editing technologies in bacteria and mammalian cells
Become proficient in DNA sequencing (Sanger and NGS)
Become proficient in designing and executing experiments
Become proficient in analyzing and visualizing data
Assist with routine laboratory tasks (organizing, ordering, stocking, etc.)
Prepare reagents and solutions
Develop and optimize genome editing assays
Record experimental details and results in an electronic notebook
Time management
Communication skills
Present summaries of work or scientific papers in lab meeting and journal club, respectively
Perform laboratory experiments following established protocols and procedures
Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines
Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed
Record experimental data accurately and maintain organized records of procedures, protocols, and results
Compile and analyze data using appropriate statistical and analytical methods
Assist in data interpretation and presentation, contributing to research findings and reports