Research Associate LT

Chapman University Careers•Irvine, CA

12d

About The Position

Under general supervision, the candidate should work effectively in a team environment and have demonstrated high productivity and reproducible technical skills, including mouse physiological assessments, mouse surgery, cell culture techniques, Western Blotting, DNA / RNA extraction and PCR , gene transfection, protein purification, and Immunofluorescence/Immunohistochemistry. This person is also expected to be detail-oriented and self-motivated, and knowledge of computers is essential for high volume research and maintenance of the data records. The candidate will be expected to present data in the weekly laboratory seminars after independent analysis and write the methodology and results for the manuscript and grant preparation. This person also provides trainings to undergraduate students and oversees their research on the day-to-day basis.

Requirements

Ph.D in the field of Biomedical Sciences, Molecular Biology, Biochemistry, Cell Biology or Pharmacy with molecular biology emphasis or its equivalent in research skill and subject matter knowledge.
Must be available to work extended hours to be able to complete time consuming experiments.
Proven experience working with rodents, and genotyping rodents
Proven practical experience with techniques of molecular biology.
Working experience with cell lines and/or primary culture
Proficient in research methods, testing and data collection, analysis, evaluation, report writing, independent discretion and judgment.
Knowledge of research and experimental methods as issued by Institutional Research Boards.
Good organizational skills and ability to prioritize and handle multiple complex tasks independently.
Ability to operate laboratory equipment and maintain a safe working environment.
Ability to conduct research in a timely manner and document all data.
Strong writing skills and computational knowledge.
Ability to establish and maintain cooperative working relationships with co-workers, investigators and other research staff.
Ability to work alone and/or with student (or laboratory) assistants.

Responsibilities

Conduct research experiments independently and in consultation with supervisor.
Perform mouse handling, tattooing, tail sampling, and genotyping.
Should be willing to work long hours for completion of long and time-consuming experiments.
Determine mouse body temperature (rectal thermometry).
Perform mouse blood collection, mouse behavior analysis, mouse organ dissection, thyroidectomy and mouse brain sectioning.
Prepare technical reports, papers, graphs and/or records and provides interpretations.
Perform PCR , Western blot, ELISA , immunostaining, immunohistochemistry, and other techniques of molecular biology as needed.
Maintain mammalian cell lines, perform transfections, and collect samples.
Maintain mamalian cell lines, perform transfections, and collect samples.
Perform bacterial transformation and plasmid purification.
Histology sample preparation, confocal microscopy, data analysis, and presentation.
Collaborate with other faculty with similar research interests on this topic.
Maintains and updates record of all experimental studies.
Stores all biochemical data in digital format on computers and backs up regularly.
Consult and assist Principal Investigator Work alongside Principal Investigator to determine method, details, and means of performing the experiments.
Furnish regular updates to Principal Investigator.
Ensure the facility is well equipped with all necessary tools to conduct research Place request orders for equipment and lab supplies Use equipment and laboratory space accordingly to work contract Contribute to the development of research documentation for publication and/or presentation.
Perform other duties as assigned.