Research Associate III

Seattle Children's • Seattle, WA

1d

About The Position

Researcher who is competent in cellular, molecular and viral procedures and have mouse colony management experiences. Will be involved in lab management like ordering and inventory maintenance. Specific techniques include molecular cloning (Gibson assembly, PCR, gel electrophoresis, restriction digest), cell culture (293T cells, cancer cell lines), DNA transfection (PEI, lipofectamine), Western Blot, Viral production (MLV, AAV), Fluorescent Activated Cell Sorting. Advanced technique including next-generation sequencing. Basic image processing and analysis. Basic statistics like statistical significance testing. Applicants should also have mouse colony management experience. Preferred experiences with softwares include Prism, Excel, Python, MATLAB, ImageJ. Will work on a project on bioengineering, in a collaborative team and help lab operations. Important to be proactive and takes initiatives and communicative. High tolerance for uncertainty and willing to take action to solve problems.

Requirements

• Bachelor’s Degree in Microbiology, Biochemistry, Immunology, or related field
• At least seven (7) years laboratory experience
• Proactive and takes initiatives and communicative
• High tolerance for uncertainty and willing to take action to solve problems

Nice To Haves

• Master’s Degree in Microbiology, Biochemistry, Immunology, or related field
• Previous research experience in biochemistry, molecular biology, and tissue culture
• Experiences with softwares include Prism, Excel, Python, MATLAB, ImageJ

Responsibilities

• Lab management like ordering and inventory maintenance
• Molecular cloning (Gibson assembly, PCR, gel electrophoresis, restriction digest)
• Cell culture (293T cells, cancer cell lines)
• DNA transfection (PEI, lipofectamine)
• Western Blot
• Viral production (MLV, AAV)
• Fluorescent Activated Cell Sorting
• Next-generation sequencing
• Basic image processing and analysis
• Basic statistics like statistical significance testing
• Mouse colony management

Benefits

• medical
• dental
• vision plans
• 403(b)
• life insurance
• paid time off
• tuition reimbursement

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level
• Number of Employees: 501-1,000 employees